Abstract
A 24-membered ring macrolide compound, macrolactin A has potential applications in pharmaceuticals for its anti-infectious and antiviral activity. In this study, macrolactin A was produced by a marine bacterium, which was identified as Bacillus subtilis by 16S ribosomal RNA (rRNA) sequence analysis. Electrospray ionization mass spectrometry (ESI/MS) and nuclear magnetic resonance (NMR) spectroscopy analyses were used to characterize this compound. To improve the production, response surface methodology (RSM) involving Box-Behnken design (BBD) was employed. Faeces bombycis, the main by-product in sericulture, was used as a nitrogen source in fermentation. The interactions between three significant factors, F. bombycis, soluble starch, and (NH4)2SO4 were investigated. A quadratic model was constructed to fit the production and the factors. Optimum medium composition was obtained by analysis of the model. When cultivated in the optimum medium, the production of macrolactin A was increased to 851 mg/L, 2.7 times as compared to the original. This study is also useful to find another way in utilizing F. bombycis.
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Project supported by the Science and Technology Department of Zhejiang Province, China (No. 2009C33019), the Industry-University-Research Institution Alliance for Microbial Medicine Technology Innovation and New Drug Development of China (No. 2010ZX090401-403), and the National Science and Technology Major Project of New Drug of China (Nos. 2011ZX09201-101 and 2012ZX09103101-075)
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Chen, H., Wu, Mb., Chen, Zj. et al. Enhancing production of a 24-membered ring macrolide compound by a marine bacterium using response surface methodology. J. Zhejiang Univ. Sci. B 14, 346–354 (2013). https://doi.org/10.1631/jzus.B1200153
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DOI: https://doi.org/10.1631/jzus.B1200153
Key words
- 24-membered ring macrolide
- Enhancing production
- Response surface methodology
- Faeces bombycis
- Marine bacterium