Skip to main content
Log in

Volatile profile analysis and quality prediction of Longjing tea (Camellia sinensis) by HS-SPME/GC-MS

  • Published:
Journal of Zhejiang University SCIENCE B Aims and scope Submit manuscript

Abstract

This study aimed to analyze the volatile chemical profile of Longjing tea, and further develop a prediction model for aroma quality of Longjing tea based on potent odorants. A total of 21 Longjing samples were analyzed by headspace solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). Pearson’s linear correlation analysis and partial least square (PLS) regression were applied to investigate the relationship between sensory aroma scores and the volatile compounds. Results showed that 60 volatile compounds could be commonly detected in this famous green tea. Terpenes and esters were two major groups characterized, representing 33.89% and 15.53% of the total peak area respectively. Ten compounds were determined to contribute significantly to the perceived aroma quality of Longjing tea, especially linalool (0.701), nonanal (0.738), (Z)-3-hexenyl hexanoate (−0.785), and β-ionone (−0.763). On the basis of these 10 compounds, a model (correlation coefficient of 89.4% and cross-validated correlation coefficient of 80.4%) was constructed to predict the aroma quality of Longjing tea. Summarily, this study has provided a novel option for quality prediction of green tea based on HS-SPME/GC-MS technique.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Alvarez, R.Q., Passaro, C.C., Lara, O.G., Londono, J.L., 2011. Relationship between chromatographic profiling by HS-SPME and sensory quality of mandarin juices: effect of squeeze technology. Proc. Food Sci., 1:1396–1403. [doi:10.1016/j.profoo.2011.09.207]

    Article  CAS  Google Scholar 

  • Augusto, F., Zini, C.A., 2002. Solid Phase Microextraction. In: Pawliszyn, J. (Ed.), Sampling and Sample Preparation for Field and Laboratory: Fundamentals and New Directions in Sample Preparation. Elsevier, Amsterdam, p.389–478.

  • Azodanlou, R., Darbellay, C., Luisier, J.L., Villettaz, J.C., Amado, R., 2003a. Quality assessment of strawberries (Fragaria species). J. Agric. Food Chem., 51(3):715–721. [doi:10.1021/jf0200467]

    Article  PubMed  CAS  Google Scholar 

  • Azodanlou, R., Darbellay, C., Luisier, J.L., Villettaz, J.C., Amadò, R., 2003b. Development of a model for quality assessment of tomatoes and apricots. LWT-Food Sci. Technol., 36(2):223–233. [doi:10.1016/s0023-6438(02)00204-9]

    Article  CAS  Google Scholar 

  • Cheng, Y., Huynh-Ba, T., Blank, I., Robert, F., 2008. Temporal changes in aroma release of Longjing tea infusion: interaction of volatile and nonvolatile tea components and formation of 2-butyl-2-octenal upon aging. J. Agric. Food Chem., 56(6):2160–2169. [doi:10.1021/jf073132l]

    Article  PubMed  CAS  Google Scholar 

  • Choi, H.S., 2003. Character impact odorants of Citrus hallabong [(C. unshiu Marcov×C. Sinensis Osbeck)×C. Reticulata Blanco] cold-pressed peel oil. J. Agric. Food Chem., 51(9):2687–2692 [doi:10.1021/jf021069o]

    Article  PubMed  CAS  Google Scholar 

  • Choi, H.S., 2005. Characteristic odor components of kumquat (Fortunella japonica Swingle) peel oil. J. Agric. Food Chem., 53(5):1642–1647. [doi:10.1021/jf040324x]

    Article  PubMed  CAS  Google Scholar 

  • General Administration of Quality Supervision, Inspection and Quarantine (AQSIQ) and the Standardization Administration of China (SAC), 2008. Product of Geographical Indication-Longjing Tea, GB/T 18650-2008. China Standard Publishing House, Beijing (in Chinese).

    Google Scholar 

  • General Administration of Quality Supervision, Inspection and Quarantine (AQSIQ) and the Standardization Administration of China (SAC), 2009. Methodology of Sensory Evaluation of Tea, GB/T 23776-2009. China Standard Publishing House, Beijing (in Chinese).

    Google Scholar 

  • Jeleń, H.H., Obuchowska, M., Zawirska-Wojtasiak, R., Wasowicz, E., 2000. Headspace solid-phase microextraction use for the characterization of volatile compounds in vegetable oils of different sensory quality. J. Agric. Food Chem., 48(6):2360–2367. [doi:10.1021/jf991095v]

    Article  PubMed  Google Scholar 

  • Jøgensen, U., Hansen, M., Christensen, L.P., Jensen, K., Kaack, K., 2000. Olfactory and quantitative analysis of aroma compounds in elder flower (Sambucus nigra L.) drink processed from five cultivars. J. Agric. Food Chem., 48(6):2376–2383. [doi:10.1021/jf000005f]

    Article  Google Scholar 

  • Jumtee, K., Komura, H., Bamba, T., Fukusaki, E., 2011. Predication of Japanese green tea (Sen-cha) ranking by volatile profiling using gas chromatography mass spectrometry and multivariate analysis. J. Biosci. Bioeng., 112(3):252–255. [doi:10.1016/j.jbiosc.2011.05.008]

    Article  PubMed  CAS  Google Scholar 

  • Kawakami, M., Yamanishi, T., 1983. Flavor constituents of longjing tea. Agric. Biol. Chem., 47(9):2077–2083. [doi:10.1271/bbb1961.47.2077]

    Article  CAS  Google Scholar 

  • Kumazawa, K., Masuda, H., 1999. Identification of potent odorants in Japanese green tea (Sen-cha). J. Agric. Food Chem., 47(12):5169–5172. [doi:10.1021/jf9906782]

    Article  PubMed  CAS  Google Scholar 

  • Kumazawa, K., Masuda, H., 2002. Identification of potent odorants in different green tea varieties using flavor dilution technique. J. Agric. Food Chem., 50(20): 5660–5663. [doi:10.1021/jf020498j]

    Article  PubMed  CAS  Google Scholar 

  • Lavilla, T., Puy, J., López, M.L., Recasens, I., Vendrell, M., 1999. Relationships between volatile production, fruit quality, and sensory evaluation in granny smith apples stored in different controlled-atmosphere treatments by means of multivariate analysis. J. Agric. Food Chem., 47(9):3791–3803. [doi:10.1021/jf990066h]

    Article  PubMed  CAS  Google Scholar 

  • Lv, H.P., Zhong, Q.S., Lin, Z., Wang, L., Tan, J.F., Guo, L., 2012. Aroma characterisation of Pu-erh tea using headspace-solid phase microextraction combined with GC/MS and GC-olfactometry. Food Chem., 130(4): 1074–1081. [doi:10.1016/j.foodchem.2011.07.135]

    Article  CAS  Google Scholar 

  • Pino, J.A., Marbot, R., Vázquez, C., 2002. Characterization of volatiles in Costa Rican guava [Psidium friedrichsthalianum (Berg) Niedenzu] fruit. J. Agric. Food Chem., 50(21):6023–6026. [doi:10.1021/jf011456i]

    Article  PubMed  CAS  Google Scholar 

  • Pongsuwan, W., Fukusaki, E., Bamba, T., Yonetani, T., Yamahara, T., Kobayashi, A., 2007. Prediction of Japanese green tea ranking by gas chromatography/mass spectrometry-based hydrophilic metabolite fingerprinting. J. Agric. Food Chem., 55(2):231–236. [doi:10.1021/jf062330u]

    Article  PubMed  CAS  Google Scholar 

  • Pongsuwan, W., Bamba, T., Yonetani, T., Kobayashi, A., Fukusaki, E., 2008. Quality prediction of Japanese green tea using pyrolyzer coupled GC/MS based metabolic fingerprinting. J. Agric. Food Chem., 56(3):744–750. [doi:10.1021/jf072791v]

    Article  PubMed  CAS  Google Scholar 

  • Shimoda, M., Shigematsu, H., Shiratsuchi, H., Osajima, Y., 1995. Comparison of volatile compounds among different grades of green tea and their relations to odor attributes. J. Agric. Food Chem., 43(6):1621–1625. [doi:10.1021/jf00054a038]

    Article  CAS  Google Scholar 

  • Togari, N., Kobayashi, A., Aishima, T., 1995. Relating sensory properties of tea aroma to gas chromatographic data by chemometric calibration methods. Food Res. Int., 28(5):485–493. [doi:10.1016/0963-9969(95)00028-3]

    Article  CAS  Google Scholar 

  • Wang, K., Ruan, J., 2009. Analysis of chemical components in green tea in relation with perceived quality, a case study with Longjing teas. Int. J. Food Sci. Technol., 44(12): 2476–2484. [doi:10.1111/j.1365-2621.2009.02040.x]

    Article  CAS  Google Scholar 

  • Wang, K., Liu, F., Liu, Z., Huang, J., Xu, Z., Li, Y., Chen, J., Gong, Y., Yang, X., 2010. Analysis of chemical components in oolong tea in relation to perceived quality. Int. J. Food Sci. Technol., 45(5):913–920. [doi:10.1111/j.1365-2621.2010.02224.x]

    CAS  Google Scholar 

  • Wold, S., Sjöström, M., Eriksson, L., 2001. PLS-regression: a basic tool of chemometrics. Chemometr. Int. Lab. Syst., 58(2):109–130. [doi:10.1016/s0169-7439(01)00155-1]

    Article  CAS  Google Scholar 

  • Yu, S.J., Zhang, G.X., 2002. The discrimination of Longjing tea. J. Chin. Tea Proc., 2:35–36 (in Chinese).

    Google Scholar 

  • Zhu, M., Li, E., He, H., 2008. Determination of volatile chemical constitutes in tea by simultaneous distillation extraction, vacuum hydrodistillation and thermal desorption. Chromatographia, 68(7–8):603–610. [doi:10.1365/s10337-008-0732-1]

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Xiao-chang Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lin, J., Dai, Y., Guo, Yn. et al. Volatile profile analysis and quality prediction of Longjing tea (Camellia sinensis) by HS-SPME/GC-MS. J. Zhejiang Univ. Sci. B 13, 972–980 (2012). https://doi.org/10.1631/jzus.B1200086

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1631/jzus.B1200086

Key words

CLC number

Navigation