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Optimization of succinic acid fermentation with Actinobacillus succinogenes by response surface methodology (RSM)

Journal of Zhejiang University SCIENCE B volume 13, pages 103–110 (2012)Cite this article

Abstract

Succinic acid is considered as an important platform chemical. Succinic acid fermentation with Actinobacillus succinogenes strain BE-1 was optimized by central composite design (CCD) using a response surface methodology (RSM). The optimized production of succinic acid was predicted and the interactive effects between glucose, yeast extract, and magnesium carbonate were investigated. As a result, a model for predicting the concentration of succinic acid production was developed. The accuracy of the model was confirmed by the analysis of variance (ANOVA), and the validity was further proved by verification experiments showing that percentage errors between actual and predicted values varied from 3.02% to 6.38%. In addition, it was observed that the interactive effect between yeast extract and magnesium carbonate was statistically significant. In conclusion, RSM is an effective and useful method for optimizing the medium components and investigating the interactive effects, and can provide valuable information for succinic acid scale-up fermentation using A. succinogenes strain BE-1.

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Affiliations

  1. School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), Beijing, 100083, China

    Yun-jian Zhang & Yu-xiu Zhang

  2. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China

    Qiang Li, Dan Wang & Jian-min Xing

Authors
  1. Yun-jian Zhang
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  2. Qiang Li
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  3. Yu-xiu Zhang
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  4. Dan Wang
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  5. Jian-min Xing
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Corresponding author

Correspondence to Yu-xiu Zhang.

Additional information

Project supported by the National Major Special Project on New Varieties Cultivation for Transgenic Organisms (No. 2009ZX08009-130B), the National Natural Science Foundation of China (No. 50874112), and the National High-Tech R&D Program (863) of China (No. 2009AA06Z320)

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Zhang, Yj., Li, Q., Zhang, Yx. et al. Optimization of succinic acid fermentation with Actinobacillus succinogenes by response surface methodology (RSM). J. Zhejiang Univ. Sci. B 13, 103–110 (2012). https://doi.org/10.1631/jzus.B1100134

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  • DOI: https://doi.org/10.1631/jzus.B1100134

Key words

  • Succinic acid
  • Response surface methodology (RSM)
  • Optimization
  • Actinobacillus succinogenes

CLC number

  • Q815