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Comparative proteome analysis of Helicobacter pylori clinical strains by two-dimensional gel electrophoresis

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Abstract

Objective

To investigate the pathogenic properties of Helicobacter pylori by comparing the proteome map of H. pylori clinical strains.

Methods

Two wild-type H. pylori strains, YN8 (isolated from biopsy tissue of a gastric cancer patient) and YN14 (isolated from biopsy tissue of a gastritis and duodenal ulcer patient), were used. Proteomic analysis, using a pH range of 3–10 and 5–8, was performed. The individual proteins were identified by quadrupole time-of-flight (Q-TOF) mass spectrometer and protein database search.

Results

Variation in spot patterns directed towards differential protein expression levels was observed between the strains. The gel revealed prominent proteins with several protein “families”. The comparison of protein expressions of the two strains reveals a high variability. Differentially present or absent spots were observed. Nine differentially expressed protein spots identified by Q-TOF included adenosine triphosphate (ATP)-binding protein, disulfide oxidoreductase B (DsbB)-like protein, N utilization substance A (NusA), ATP-dependent protease binding subunit/heat shock protein, hydantoin utilization protein A, seryl-tRNA synthetase, molybdenum ABC transporter ModD, and hypothetical proteins.

Conclusions

This study suggests that H. pylori strains express/repress protein variation, not only in terms of the virulence proteins, but also in terms of physiological proteins, when they infect a human host. The difference of protein expression levels between H. pylori strains isolated from gastric cancer and gastritis may be the initiator of inflammation, and result in the different clinical presentation. In this preliminary study, we report seven differential proteins between strains, with molecule weights from approximately 10 kDa to approximately 40 kDa. Further studies are needed to investigate those proteins and their function associated with H. pylori colonization and adaptation to host environment stress.

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Authors and Affiliations

  1. Department of Laboratory, Beijing Tiantan Hospital Affiliated to Capital Medical University, Beijing, 100050, China

    Ya-nan Zhang

  2. Department of Gastroenterology, Peking University Third Hospital, Beijing, 100191, China

    Shi-gang Ding, Jing Zhang & Yan-yan Shi

  3. Department of Thoracic and Cardiovascular Surgery, Beijing Shi Jing Shan Hospital, Beijing, 100043, China

    Liu-huan Huang

  4. Peking University Health Science Center, Beijing, 100091, China

    Li-jun Zhong

Authors
  1. Ya-nan Zhang
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  2. Shi-gang Ding
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  3. Liu-huan Huang
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  4. Jing Zhang
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  5. Yan-yan Shi
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  6. Li-jun Zhong
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Correspondence to Shi-gang Ding.

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Project supported by the National Natural Science Foundation of China (No. 30770980), and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (2008)

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Zhang, Yn., Ding, Sg., Huang, Lh. et al. Comparative proteome analysis of Helicobacter pylori clinical strains by two-dimensional gel electrophoresis. J. Zhejiang Univ. Sci. B 12, 820–827 (2011). https://doi.org/10.1631/jzus.B1000445

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  • DOI: https://doi.org/10.1631/jzus.B1000445

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