Abstract
A high-performance liquid chromatography (HPLC) system was used in the reversed phase mode for the determination of benzalkonium chloride (BKC) in azithromycin viscous ophthalmic drops. A Venusil-XBP(L)-C18 (150 mm×4.6 mm, 5 µm) column was used at 50 °C. The mobile phase consisted of a mixture of methanol-potassium phosphate (16:5, v/v). Two sample preparation methods were compared. The results suggested that, compared with an extraction procedure, a deproteinization procedure was much quicker and more convenient. Using the deproteinization procedure for sample preparation, calibration curves were linear in the range 5.0∼50 µg/ml. The within-day and inter-day coefficients of variation were less than 10%. The average recoveries were determined as 96.70%, 98.52%, and 97.96% at concentrations of 10.0, 30.0, and 50.0 µg/ml, respectively. Variability in precision did not exceed 5%. In conclusion, this HPLC method using a simple sample treatment procedure appears suitable for monitoring BKC content in azithromycin viscous ophthalmic drops.
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Project (No. 2008BAI55B03) supported by the Key Project of the National Science and Technology Pillar Program, China
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Shen, Y., Xu, Sj., Wang, Sc. et al. Determination of benzalkonium chloride in viscous ophthalmic drops of azithromycin by high-performance liquid chromatography. J. Zhejiang Univ. Sci. B 10, 877–882 (2009). https://doi.org/10.1631/jzus.B0920229
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DOI: https://doi.org/10.1631/jzus.B0920229