Abstract
We examined salt tolerance responsive genes in Pak-choi under salt stress and analyze their potential function. The mRNA differential display was used to screen the transcript derived fragments (TDFs) related to salinity tolerance in tolerant and moderately tolerant Pak-choi germplasm. Seventy-eight primer combinations generated 101 differential cDNA fragments, which were divided into 10 expression types. Seven cDNA sequences (GenBank accession Nos. DQ006915∼DQ006921) obtained and sequenced were highly homologous to some known expression genes or the genes related to the signaling pathways in plants under different abiotic stress.
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Project supported by the National “the Tenth Five-Year-Plan” Key Program (No. 2004BA525B08) of China and the Key Laboratory of Vegetable Genetics and Physiology, Ministry of Agriculture, China
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Qiu, Y., Li, Xx., Zhi, Hy. et al. Differential expression of salt tolerance related genes in Brassica campestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee. J. Zhejiang Univ. Sci. B 10, 847–851 (2009). https://doi.org/10.1631/jzus.B0920098
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DOI: https://doi.org/10.1631/jzus.B0920098
Key words
- Brassica campestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee
- Salinity tolerance
- Gene differential expression
- cDNA fragments
- Basic local alignment search tool (BLAST) analysis