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A non-labeled DNA biosensor based on light addressable potentiometric sensor modified with TiO2 thin film

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Abstract

Titanium dioxide (TiO2) thin film was deposited on the surface of the light addressable potentiometric sensor (LAPS) to modify the sensor surface for the non-labeled detection of DNA molecules. To evaluate the effect of ultraviolet (UV) treatment on the silanization level of TiO2 thin film by 3-aminopropyltriethoxysilane (APTS), fluorescein isothiocyanate (FITC) was used to label the amine group on the end of APTS immobilized onto the TiO2 thin film. We found that, with UV irradiation, the silanization level of the irradiated area of the TiO2 film was improved compared with the non-irradiated area under well-controlled conditions. This result indicates that TiO2 can act as a coating material on the biosensor surface to improve the effect and efficiency of the covalent immobilization of biomolecules on the sensor surface. The artificially synthesized probe DNA molecules were covalently linked onto the surface of TiO2 film. The hybridization of probe DNA and target DNA was monitored by the recording of I-V curves that shift along the voltage axis during the process of reaction. A significant LAPS signal can be detected at 10 µmol/L of target DNA sample.

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Authors and Affiliations

  1. Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China

    Xiao-lin Zong, Chun-sheng Wu, Xiao-ling Wu, Yun-feng Lu & Ping Wang

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  1. Xiao-lin Zong
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  2. Chun-sheng Wu
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  3. Xiao-ling Wu
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  4. Yun-feng Lu
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  5. Ping Wang
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Correspondence to Ping Wang.

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Project supported by the National Natural Science Foundation of China (Nos. 30627002 and 60725102), and the Interdisciplinary Research Foundation of Zhejiang University (No. 2009-15), China

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Zong, Xl., Wu, Cs., Wu, Xl. et al. A non-labeled DNA biosensor based on light addressable potentiometric sensor modified with TiO2 thin film. J. Zhejiang Univ. Sci. B 10, 860–866 (2009). https://doi.org/10.1631/jzus.B0920090

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  • DOI: https://doi.org/10.1631/jzus.B0920090

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