Optimization of antioxidant activity by response surface methodology in hydrolysates of jellyfish (Rhopilema esculentum) umbrella collagen
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To optimize the hydrolysis conditions to prepare hydrolysates of jellyfish umbrella collagen with the highest hydroxyl radical scavenging activity, collagen extracted from jellyfish umbrella was hydrolyzed with trypsin, and response surface methodology (RSM) was applied. The optimum conditions obtained from experiments were pH 7.75, temperature (T) 48.77 °C, and enzyme-to-substrate ratio ([E]/[S]) 3.50%. The analysis of variance in RSM showed that pH and [E]/[S] were important factors that significantly affected the process (P<0.05 and P<0.01, respectively). The hydrolysates of jellyfish umbrella collagen were fractionated by high performance liquid chromatography (HPLC), and three fractions (HF-1>3000 Da, 1000 Da<HF-2<3000 Da, and HF-3<1000 Da) were collected. The HF-2 fraction had the highest hydroxyl radical scavenging activity with the highest yield compared with the other two fractions. Furthermore, HF-2 also showed the strongest Cu2+-chelating ability and the best tyrosinase-inhibitory activity.
Key wordsJellyfish umbrella collagen Hydrolysis Antioxidant activity Response surface methodology (RSM)
CLC numberS98 TS254
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- Box, G., Hunter, W.G., Hunter, J.S., 1978. Statistics for Experimenters. John Wiley and Sons Inc., New York.Google Scholar
- Curto, E.V., Kwong, C., Hermersdörfer, H., Glatt, H., Santis, C., Virador, V., 1999. Inhibitors of mammalian melanocyte tyrosinase: in vitro comparisons of alkyl esters of gentisic acid with other putative inhibitors. Biochemical Pharmacology, 57(6):663–672. [doi:10.1016/S0006-2952(98)00340-2]PubMedCrossRefGoogle Scholar
- Guerard, F., Sumaya-Martinez, M.T., Laroque, D., Chabeaud, A., Dufosse, L., 2007. Optimization of free radical scavenging activity by response surface methodology in the hydrolysis of shrimp processing discards. Process Biochemistry, 42(11):1486–1491. [doi:10.1016/j.procbio.2007.07.016]CrossRefGoogle Scholar
- Klompong, V., Benjakul, S., Kantachote, D., Shahidi, F., 2007. Antioxidative activity and functional properties of protein hydrolysate of yellow stripe trevally (Selaroides leptolepis) as influenced by the degree of hydrolysis and enzyme type. Food Chemistry, 102(4):1317–1327. [doi:10.1016/j.foodchem.2006.07.016]CrossRefGoogle Scholar
- Ren, J.Y., Zhao, M.M., Shi, J., Wang, J.S., JIang, Y.M., Cui, C., Kakuda, Y., Xue, S.J., 2008. Optimization of antioxidant peptide production from grass carp sarcoplasmic protein using response surface methodology. Food Science and Technology, 41(9):1624–1632. [doi:10.1016/j.lwt. 2007.11.005]Google Scholar
- Tamaru, S., Kurayama, T., Sakono, M., Fukuda, N., Nakamori, T., Furuta, H., Tanaka, K., Sugano, M., 2007. Effects of dietary soybean peptides on hepatic production of ketone bodies and secretion of triglyceride by perfused rat liver. Bioscience, Biotechnology, Biochemistry, 71(10):2451–2457. [doi:10.1271/bbb.70221]CrossRefGoogle Scholar
- Xie, L.P., Chen, Q.X., Huang, H., Liu, X.D., Chen, H.T., Zhang, R.Q., 2003. Inhibitory effects of cupferron on the monophenolase and diphenolase activity of mushroom tyrosinase. The International Journal of Biochemistry & Cell Biology, 35(12):1658–1666. [doi:10.1016/S1357-2725(03)00006-2]CrossRefGoogle Scholar
- Zhao, X., Xue, C.H., Li, Z.J., Cai, Y.P., Liu, H.Y., Qi, H.T., 2004. Antioxidant and hepato protective activities of low molecular weight sulfated polysaccharide from Laminaria japonica. Journal of Applied Physics, 16(2): 111–115. [doi:10.1023/B:JAPH.0000044822.10744.59]Google Scholar
- Zhou, J., Hu, N., Wu, Y. L., Pan, Y.J., Sun, C.R., 2008. Preliminary studies on the chemical characterization and antioxidant properties of acidic polysaccharides from Sargassum fusiforme. Journal of Zhejiang University SCIENCE B, 9(9):721–727. [doi:10.1631/jzus.B0820025]PubMedCrossRefGoogle Scholar