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Effects of 60-day NO2 fumigation on growth, oxidative stress and antioxidative response in Cinnamomum camphora seedlings

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Abstract

Objective

To study the oxidative stress and antioxidative response of Cinnamomum camphora seedlings exposed to nitrogen dioxide (NO2) fumigation.

Methods

Measurements were made up of the growth, chlorophyll content, chlorophyll fluorescence, antioxidant system and lipid peroxidation of one-year-old C. camphora seedlings exposed to NO2 (0.1, 0.5, and 4 μl/L) fumigation in open top chambers over a period of 60 d.

Results

After the first 30 d, 0.5 and 4.0 μl/L NO2 showed insignificant effects on the growth of C. camphora seedlings. However, exposure to 0.5 and 4.0 μl/L NO2 for 15 d significantly reduced their chlorophyll content (P<0.05), enhanced their malondialdehyde (MDA) content and superoxide dismutase (SOD) activity (P<0.05), and also significantly reduced the maximal quantum yield of PSII in the dark [the ratio of variable fluorescence to maximal fluorescence (F v/F m)] (P<0.05). In the latter 30 d, 0.5 μl/L NO2 showed a positive effect on the vitality of the seedlings, which was reflected by a recovery in the ratio of F v/F m and chlorophyll content, and obviously enhanced growth, SOD activity, ascorbate (AsA) content and glutathione reductase (GR) activity (P<0.05); 4.0 μl/L NO2 then showed a negative effect, indicated by significant reductions in chlorophyll content and the ratio of F v/F m, and inhibited growth (P<0.05). Conclusion: The results suggest adaptation of C. camphora seedlings to 60-d exposure to 0.1 and 0.5 μl/L NO2, but not to 60-d exposure to 4.0 μl/L NO2. C. camphora seedlings may protect themselves from injury by strengthening their antioxidant system in response to NO2-induced oxidative stress.

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Authors and Affiliations

  1. College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310029, China

    Zhuo-mei Chen & Ying-xu Chen

  2. Zhejiang Forestry Academy, Hangzhou, 310023, China

    Zhuo-mei Chen, Guo-jian Du & Feng Li

  3. Department of Geography, Minjiang University, Fuzhou, 350108, China

    Xi-lin Wu

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  1. Zhuo-mei Chen
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  2. Ying-xu Chen
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  5. Feng Li
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Correspondence to Ying-xu Chen.

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Project supported by Zhejiang Keystone Projects (No. 2005C22056), and the Zhejiang Provincial Natural Science Foundation of China (No. Y5080011)

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Chen, Zm., Chen, Yx., Du, Gj. et al. Effects of 60-day NO2 fumigation on growth, oxidative stress and antioxidative response in Cinnamomum camphora seedlings. J. Zhejiang Univ. Sci. B 11, 190–199 (2010). https://doi.org/10.1631/jzus.B0910350

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