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Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide

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Abstract

Endothelial cell death due to increased reactive oxygen species (ROS) may contribute to the initial endothelial injury, which promotes atherosclerotic lesion formation. Piper sarmentosum (PS), a natural product, has been shown to have an antioxidant property, which is hypothesized to inhibit production of ROS and prevent cell injury. Thus, the present study was designed to determine the effects of PS on the hydrogen peroxide (H2O2)-induced oxidative cell damage in cultured human umbilical vein endothelial cells (HUVECs). In this experiment, HUVECs were obtained by collagenase perfusion of the large vein in the umbilical cord and cultured in medium M200 supplemented with low serum growth supplementation (LSGS). HUVECs were treated with various concentrations of H2O2 (0–1000 μmol/L) and it was observed that 180 μmol/L H2O2 reduced cell viability by 50% as denoted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Using the above concentration as the positive control, the H2O2-induced HUVECs were concomitantly treated with various concentrations (100, 150, 250 and 300 μg/ml) of three different extracts (aqueous, methanol and hexane) of PS. Malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) levels showed a significant increase (P<0.05) in HUVECs compared to the negative control. However, PS extracts showed a protective effect on HUVECs from H2O2-induced cell apoptosis with a significant reduction in MDA, SOD, CAT and GPX levels (P<0.05). Furthermore, PS had exhibited ferric reducing antioxidant power with its high phenolic content. Hence, it was concluded that PS plays a beneficial role in reducing oxidative stress in H2O2-induced HUVECs.

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Authors and Affiliations

  1. Department of Physiology, Universiti Kebangsaan Malaysia Medical Centre, Jalan Raja Muda Aziz, 50300, Kuala Lumpur, Malaysia

    Abdul Hamid Hafizah, Zakaria Zaiton & Megat Mohd Nordin Nor Anita

  2. Department of Obstetrics and Gynecology, Universiti Kebangsaan Malaysia Medical Centre, 56000, Cheras, Kuala Lumpur, Malaysia

    Abdullah Mahdy Zaleha

  3. Department of Human Anatomy, Division of Physiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, 43400, Selangor, Malaysia

    Abdul Hamid Hafizah & Amom Zulkhairi

  4. Forest Research Institute of Malaysia, Kepong, 52109, Selangor, Malaysia

    Adenan Mohd Ilham

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  1. Abdul Hamid Hafizah
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  2. Zakaria Zaiton
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Correspondence to Zakaria Zaiton.

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Project (Nos. UKM-FF-03-FRGS0005-2007 and FF-138-2007) supported by the Ministry of Higher Education and Universiti Kebangsaan Malaysia

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Hafizah, A.H., Zaiton, Z., Zulkhairi, A. et al. Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide. J. Zhejiang Univ. Sci. B 11, 357–365 (2010). https://doi.org/10.1631/jzus.B0900397

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