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Overexpression of the steroidogenic acute regulatory protein increases the expression of ATP-binding cassette transporters in microvascular endothelial cells (bEnd.3)

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Journal of Zhejiang University SCIENCE B Aims and scope Submit manuscript

Abstract

Objective

To determine the effect of steroidogenic acute regulatory protein (StAR) overexpression on the levels of adenosine triphosphate (ATP)-binding cassette transporter A1 (ABCA1) and ATP-binding cassette transporter G1 (ABCG1) in an endothelial cell line (bEnd.3).

Methods

The StAR gene was induced in bEnd.3 cells with adenovirus infection. The infection efficiency was detected by fluorescence activated cell sorter (FACS) and fluorescence microscopy. The expressions of StAR gene and protein levels were detected by real-time polymerase chain reaction (PCR) and Western blot. The gene and protein levels of ABCA1 and ABCG1 were detected by real-time PCR and Western blot after StAR overexpression.

Results

The result shows that StAR was successfully overexpressed in bEnd.3 cells by adenovirus infection. The mRNA and protein expressions of ABCA1 and ABCG1 were greatly increased by StAR overexpression in bEnd.3 cells.

Conclusion

Overexpression of StAR increases ABCA1 and ABCG1 expressions in endothelial cells.

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Authors and Affiliations

  1. Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai, 200032, China

    Yan-xia Ning, Feng-di Zhao & Lian-hua Yin

  2. Department of Medicine, Virginia Commonwealth University, Richmond, Virginia, 23298, USA

    Shun-lin Ren

  3. Veterans Affairs Medical Center, Richmond, Virginia, 23249, USA

    Shun-lin Ren

Authors
  1. Yan-xia Ning
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  2. Shun-lin Ren
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  3. Feng-di Zhao
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  4. Lian-hua Yin
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Correspondence to Lian-hua Yin.

Additional information

Project (Nos. 30871021 and 30900716) supported by the National Natural Science Foundation of China

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Ning, Yx., Ren, Sl., Zhao, Fd. et al. Overexpression of the steroidogenic acute regulatory protein increases the expression of ATP-binding cassette transporters in microvascular endothelial cells (bEnd.3). J. Zhejiang Univ. Sci. B 11, 350–356 (2010). https://doi.org/10.1631/jzus.B0900369

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  • DOI: https://doi.org/10.1631/jzus.B0900369

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