Abstract
A high-performance liquid chromatographic (HPLC) method with ultraviolet (UV) detector had been developed for simultaneous quantification of danshensu, protocatechuic aldehyde, caffeic acid, salvianolic acid D, rosmarinic acid, salvianolic acid B and salvianolic acid A in Danshen injection. According to the UV spectra of these components, three detection wavelengths have been selected as follows: 280 nm for danshensu and protocatechuic aldehyde, 326 nm for caffeic acid, salvianolic acid D and rosmarinic acid, 286 nm for salvianolic acid B and salvianolic acid A. The limit of detection (LOD) was improved to be in the range of 0.008∼0.160 μg/ml. Moreover, excellent linear behavior over the investigated concentration range was observed, with R>0.999 for all the analytes.
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Project supported by the National Basic Research Program (973) of China (No. 2005CB523402) and the Program for the New Century Excellent Talents in University of China (No. NCET-06-0515)
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Xu, Jz., Shen, J., Cheng, Yy. et al. Simultaneous detection of seven phenolic acids in Danshen injection using HPLC with ultraviolet detector. J. Zhejiang Univ. Sci. B 9, 728–733 (2008). https://doi.org/10.1631/jzus.B0820095
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DOI: https://doi.org/10.1631/jzus.B0820095
Key words
- High-performance liquid chromatography (HPLC)
- Danshen injection (DSI)
- Phenolic acids
- Multi-wavelengths
- Quantification