Skip to main content
SpringerLink
Log in

Effects of fish protein hydrolysate on growth performance and humoral immune response in large yellow croaker (Pseudosciaena crocea R.)

  • Published:
Journal of Zhejiang University SCIENCE B Aims and scope Submit manuscript

Abstract

We investigated the effects of fish protein hydrolysate (FPH) on growth performance and humoral immune response of the large yellow croaker (Pseudosciaena crocea R.). One thousand and two hundred large yellow croakers [initial average weight: (162.75±23.85) g] were divided into four groups and reared in floating sea cages (3 m×3 m×3 m). The animals were fed with 4 diets: basal diet only (control) or diets supplemented with 5%, 10% and 15% (w/w) FPH. The results show that dietary FPH levels significantly influenced the growth and immunity of the large yellow croaker. Compared with the control group, total weight gain (TWG) in all treatment groups, relative weight gain (RWG) and specific growth rate (SGR) in fish fed with diets supplemented with 10% and 15% FPH were significantly increased (P<0.05). Similar results were observed in immune parameters [lysozyme activity, serum complements, immunoglobulin M (IgM)]. Lysozyme activity, complement C4 and IgM were also significantly increased (P<0.05) in fish fed with diets supplemented with 10% and 15% FPH, while complement C3 level was significantly increased (P<0.05) in all treatment groups. In general, with the supplementation of FPH, particularly at dose of 10%, the growth performance and immunity of the large yellow croaker can be improved effectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adibi, S.A., 1997. The oligopeptide transporter (Pept-1) in human intestine: biology and function. Gastroenterology, 113(1):332–340. [doi:10.1016/S0016-5085(97)70112-4]

    Article  PubMed  CAS  Google Scholar 

  • Adler-Nissen, J., 1986. Enzymatic Hydrolysis of Food Proteins. Elsevier Applied Science Publishers, New York.

    Google Scholar 

  • Aguila, J., Cuzon, G., Pascual, C., Dominguesd, P.M., Gaxiolac, G., Sánchezc, A., Maldonadoe, T., Rosas, C., 2007. The effects of fish hydrolysate (CPSP) level on Octopus maya (Voss and Solis) diet: digestive enzyme activity, blood metabolites, and energy balance. Aquaculture, 273(4):641–655. [doi:10.1016/j.aquaculture.2007.07.010]

    Article  CAS  Google Scholar 

  • Alcorn, S.W., Murray, A.L., Pascho, R.J., 2002. Effects of rearing temperature on immune functions in sockeye salmon (Oncorhynchus nerka). Fish Shellfish Immunol., 12(4):303–334. [doi:10.1006/fsim.2001.0373]

    Article  PubMed  CAS  Google Scholar 

  • Bachère, E., 2000. Shrimp immunity and disease control. Aquaculture, 191(1–3):3–11. [doi:10.1016/S0044-8486(00)00413-0]

    Article  Google Scholar 

  • Bagni, M., Archetti, L., Amadori, M., Marino, G., 2000. Effect of long-term administration of an immunostimulant diet on innate immunity in sea bass (Dicentrarchus labrax). J. Vet. Med. B, 47(10):745–751. [doi:10.1046/j.1439-0450.2000.00412.x]

    Article  CAS  Google Scholar 

  • Berge, G.M., Storebakken, T., 1996. Fish protein hydrolyzate in starter diets for Atlantic salmon (Salmo salar) fry. Aquaculture, 145(1–4):205–212. [doi:10.1016/S0044-8486(96)01355-5]

    Article  CAS  Google Scholar 

  • Bøgwald, J., Dalmo, R., Leifson, R.M., Stenbern, E., GildBerg, A., 1996. The stimulatory effect of a muscle protein hydrolysate from Atlantic cod, Gadus morhua L. on Atlantic salmon, Salmo salar L., head kidney leucocytes. Fish Shellfish Immunol., 6(1):3–16. [doi:10.1006/fsim.1996.0002]

    Article  Google Scholar 

  • Cahu, C.L., Zambonino Infante, J.L., Quazuguel, P., Le Gall, M.M., 1999. Protein hydrolysate vs. fish meal in compound diets for 10-day old sea bass Dicentrarchus labrax larvae. Aquaculture, 171(1–2):109–119. [doi:10.1016/S0044-8486(98)00428-1]

    Article  CAS  Google Scholar 

  • Carvalho, A.P., Escaffre, A.M., Oliva Teles, A., Bergot, P., 1997. First feeding of common carp larvae on diets with high levels of protein hydrolysates. Aquac. Int., 5(4):361–367. [doi:10.1023/A:1018368208323]

    Article  Google Scholar 

  • Cook, M.T., Hayball, P.J., Hutchinson, W., Nowak, B.F., Hayball, J.D., 2003. Administration of a commercial immunostimulant preparation, EcoActiva™ as a feed supplement enhances macrophage respiratory burst and the growth rate of snapper (Pagrus auratus, Sparidae (Bloch and Schneider)) in winter. Fish Shellfish Immunol., 14(4):333–345. [doi:10.1006/fsim.2002.0441]

    Article  PubMed  CAS  Google Scholar 

  • Cuesta, A., Meseguer, J., Esteban, M.A., 2004. Total serum immunoglobulin M levels are affected by immunomodulators in seabream (Sparus aurata L.). Vet. Immunol. Immunopathol., 101(3–4):203–210. [doi:10.1016/j.vetimm.2004.04.021]

    Article  PubMed  CAS  Google Scholar 

  • Duan, Q., Mai, K., Zhong, H., Si, L., Wang, X., 2001. Studies on the nutrition of the large yellow croaker, Pseudosciaena crocea R. I: growth response to graded levels of dietary protein and lipid. Aquac. Res., 32(Suppl. 1):46–52. [doi:10.1046/j.1355-557x.2001.00048.x]

    Article  CAS  Google Scholar 

  • Duarte, J., Vinderola, G., Ritz, B., Perdigón, G., Matar, C., 2006. Immunomodulating capacity of commercial fish protein hydrolysate for diet supplementation. Immunobiology, 211(5):341–350. [doi:10.1016/j.imbio.2005.12.002]

    Article  PubMed  CAS  Google Scholar 

  • Ellis, A.E., 1990. Lysozyme Assays. In: Stolen, J.S., Fletcher, D.P., Anderson, B.S., Roberson, B.S. (Eds.), Techniques in Fish Immunology. SOS Publications, Fair Haven, USA, p.101–103.

    Google Scholar 

  • Gasque, P., 2004. Complement: a unique innate immune sensor for danger signals. Mol. Immunol., 41(11):1089–1098. [doi:10.1016/j.molimm.2004.06.011]

    Article  PubMed  CAS  Google Scholar 

  • Holland, M.C.H., Lambris, J.D., 2002. The complement system in teleosts. Fish Shellfish Immunol., 12(5):399–420. [doi:10.1006/fsim.2001.0408]

    Article  PubMed  CAS  Google Scholar 

  • Klompong, V., Benjakul, S., Kantachote, D., Shahidi, F., 2007. Antioxidative activity and functional properties of protein hydrolysate of yellow stripe trevally (Selaroides leptolepis) as influenced by the degree of hydrolysis and enzyme type. Food Chem., 102(4):1317–1327. [doi:10.1016/j.foodchem.2006.07.016]

    Article  CAS  Google Scholar 

  • Kolkovski, S., Tandler, A., 2000. The use of squid protein hydrolysate as a protein source in microdiets for gilthead seabream Sparus aurata larvae. Aquac. Nutr., 6(1):11–15. [doi:10.1046/j.1365-2095.2000.00125.x]

    Article  CAS  Google Scholar 

  • Kotzamanis, Y.P., Gisbert, E., Gatesoupe, F.J., Zambonino Infante, J., Cahu, C., 2007. Effects of different dietary levels of fish protein hydrolysates on growth, digestive enzymes, gut microbiota, and resistance to Vibrio anguillarum in European sea bass (Dicentrarchus labrax) larvae. Comp. Biochem. Physiol., Part A Mol. Integr. Physiol., 147(1):205–214. [doi:10.1016/j.cbpa.2006.12.037]

    Article  PubMed  CAS  Google Scholar 

  • Liang, M., Wang, J., Chang, Q., Mai, K., 2006. Effects of different levels of fish protein hydrolysate in the diet on the nonspecific immunity of Japanese sea bass, Lateolabrax japonicus (Cuvieret Valenciennes, 1928). Aquac. Res., 37(1):102–106. [doi:10.1111/j.1365-2109.2005.01392.x]

    Article  CAS  Google Scholar 

  • Liaset, B., Espe, M., 2008. Nutritional composition of soluble and insoluble fractions obtained by enzymatic hydrolysis of fish-raw materials. Process Biochem., 43(1):42–48. [doi:10.1016/j.procbio.2007.10.007]

    Article  CAS  Google Scholar 

  • Mai, K., Wan, J., Ai, Q., Xu, W., Liufu, Z., Zhang, L., Zhang, C., Li, H., 2006. Dietary methionine requirement of large yellow croaker, Pseudosciaena crocea R. Aquaculture, 253(1–4):564–572. [doi:10.1016/j.aquaculture.2005.08.010]

    Article  CAS  Google Scholar 

  • Murray, A.L., Ponald, J.P., Alcorn, S.W., Fairgrieve, W.T., Shearer, K.D., Roley, D., 2003. Effects of various feed supplements containing fish protein hydrolysate or fish processing by products on the innate immune functions of juvenile coho salmon (Oncorhynchus kisutch). Aquaculture, 220(1–4):643–653. [doi:10.1016/S0044-8486(02)00426-X]

    Article  CAS  Google Scholar 

  • Oliva-Teles, A., Cerqueira, A.L., Goncalves, P., 1999. The utilization of diets containing high levels of fish protein hydrolysate by turbot (Scophthalamus maximus) juveniles. Aquaculture, 179(1–4):195–201. [doi:10.1016/S0044-8486(99)00162-3]

    Article  Google Scholar 

  • Ouellet, D.R., Seoane, J.R., Veira, D.M., Proulx, J.G., 1997. Effects of supplementation with fish meal or fish protein hydrolysate on growth, nutrient digestibility and rumen fermentation of growing cattle fed grass silage. Anim. Feed Sci. Technol., 68(3–4):307–326. [doi:10.1016/S0377-8401(97)00035-7]

    Article  Google Scholar 

  • Pascual, C., Zenteno, E., Cuzon, G., Sánchez, A., Gaxiola, G., Taboada, G., Suárez, J., Maldonado, T., Rosas, C., 2004. Litopenaeus vannamei juveniles energetic balance and immunological response to dietary protein. Aquaculture, 236(1–4):431–450. [doi:10.1016/j.aquaculture.2004.01.015]

    Article  CAS  Google Scholar 

  • Puangkaew, J., Kiron, V., Somamoto, T., Okamoto, N., Satoh, S., Takeuchi, T., Watanabe, T., 2004. Nonspecific immune response of rainbow trout (Oncorhynchus mykiss Walbaum) in relation to different status of vitamin E and highly unsaturated fatty acids. Fish Shellfish Immunol., 16(1):25–39. [doi:10.1016/S1050-4648(03)00028-7]

    Article  PubMed  CAS  Google Scholar 

  • Refstie, S., Olli, J.J., Standal, H., 2004. Feed intake, growth, and protein utilisation by post-smolt Atlantic salmon (Salmo salar) in response to graded levels of fish protein hydrolysate in the diet. Aquaculture, 239(1–4):331–349. [doi:10.1016/j.aquaculture.2004.06.015]

    Article  CAS  Google Scholar 

  • Ross, D.A., Wilson, M.R., Miller, N.W., Clem, L.W., Warr, G.W., 1998. Evolutionary variation of immunoglobulin μ-heavy chain RNA processing pathways: origins, effects, and implications. Immunol. Rev., 166(1):143–151. [doi:10.1111/j.1600-065X.1998.tb01259.x]

    Article  PubMed  CAS  Google Scholar 

  • Siwicki, A.K., Anderson, D.P., 1993. Nonspecific Defense Mechanisms Assay in Fish. II. Potential Killing Activity of Neutrophils and Macrophages, Lysozyme Activity in Serum and Organs and Total Immunoglobulin Level in Serum. In: Siwicki, A.K., Anderson, D.P., Waluga, J. (Eds.), Fish Disease Diagnosis and Prevention Methods. Olsztyn, Poland, p.105–112.

    Google Scholar 

  • Song, Z., Wu, T., Cai, L., Zhang, L., Zheng, X., 2006. Effects of dietary supplementation with Clostridium butyricum on the growth performance and humoral immune response in Miichthys miiuy. J. Zhejiang Univ. Sci. B, 7(7):596–602. [doi:10.1631/jzus.2006.B0596]

    Article  PubMed  Google Scholar 

  • Tesser, M.B., Terjesen, B.F., Zhang, Y., Portella, M.C., Dabrowski, K., 2005. Free-and peptide-based dietary arginine supplementation for the South American fish pacu (Piaractus mesopotamicus). Aquac. Nutr., 11(6):443–453. [doi:10.1111/j.1365-2095.2005.00373.x]

    Article  CAS  Google Scholar 

  • Thiansilakul, Y., Benjakul, S., Shahidi, F., 2007. Compositions, functional properties and antioxidative activity of protein hydrolysates prepared from round scad (Decapterus maruadsi). Food Chem., 103(4):1385–1394. [doi:10.1016/j.foodchem.2006.10.055]

    Article  CAS  Google Scholar 

  • Watts, M., Munday, B.L., Burke, C.M., 2001. Isolation and partial characterisation of immunoglobulin from southern bluefin tuna Thunnus maccoyii Castelnau. Fish Shellfish Immunol., 11(6):491–503. [doi:10.1006/fsim.2000.0329]

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, Zhejiang University, Hangzhou, 310027, China

    Hong-gang Tang, Tian-xing Wu, Zhan-yu Zhao & Xiao-dong Pan

  2. Key Laboratory for Molecular Design and Nutrition Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo, 315100, China

    Hong-gang Tang & Tian-xing Wu

Authors
  1. Hong-gang Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tian-xing Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhan-yu Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiao-dong Pan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tian-xing Wu.

Additional information

Project (No. 2006C12098) supported by the Science and Technology Department of Zhejiang Province, China

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tang, Hg., Wu, Tx., Zhao, Zy. et al. Effects of fish protein hydrolysate on growth performance and humoral immune response in large yellow croaker (Pseudosciaena crocea R.). J. Zhejiang Univ. Sci. B 9, 684–690 (2008). https://doi.org/10.1631/jzus.B0820088

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1631/jzus.B0820088

Key words

CLC number

Search

Navigation