Abstract
To explore the applicability of anoxic-oxic (A/O) activated sludge process for petrochemical wastewater treatment, the relationship between bacterial community structure and pollutants loading/removal efficiencies was investigated by gas chromatograph-mass spectrometry (GC-MS), polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and other conventional techniques. It showed that when the concentrations of the influent chemical oxygen demand (COD) and ammonia nitrogen (NH +4 -N) were 420∼560 mg/L and 64∼100 mg/L, respectively, the corresponding average effluent concentrations were 160 mg/L and 55 mg/L, which were 1.6 and 2.2 times higher than those of the national standards in China, respectively, demonstrating the inefficient performances of A/O process. Analysis of GC-MS indicated that refractory pollutants were mainly removed by sludge adsorption, but not by biodegradation. PCR-DGGE profile analysis suggested that the biological system was species-rich, but there was apparent succession of the bacterial community structure in different locations of the A/O system. Variations of bacterial community structure and pollutant loadings had obvious influences on pollutants removal efficiencies. Thus, A/O process was inapplicable for the treatment of complicated petrochemical wastewater, and strategies such as the reinforcement of pre-treatment and two-stage A/O process were suggested.
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Project supported by the National Basic Research Program (973) of China (No. 2004CB418505) and the Science and Technology Development Program of Heilongjiang Province (No. CC05S301), China
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Zhao, Lj., Ma, F. & Guo, Jb. Applicability of anoxic-oxic process in treating petrochemical wastewater. J. Zhejiang Univ. Sci. A 10, 133–141 (2009). https://doi.org/10.1631/jzus.A0820006
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DOI: https://doi.org/10.1631/jzus.A0820006
Key words
- Petrochemical wastewater
- Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE)
- Anoxic-oxic (A/O) process
- Applicability