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Chemical composition and antibacterial activity of Gongronema latifolium

Abstract

Chemical composition of Gongronema latifolium leaves was determined using standard methods. Aqueous and methanol G. latifolium extracts were tested against thirteen pathogenic bacterial isolates. Crude protein, lipid extract, ash, crude fibre and nitrogen free extractives obtained are: 27.2%, 6.07%, 11.6%, 10.8% and 44.3% dry matter respectively. Potassium, sodium, calcium, phosphorus and cobalt contents are 332, 110, 115, 125 and 116 mg/kg respectively. Dominant essential amino acids are leucine, valine and phenylalanine. Aspartic acid, glutamic acid and glycine are 13.8%, 11.9% and 10.3% respectively of total amino acid. Saturated and unsaturated fatty acids are 50.2% and 39.4% of the oil respectively. Palmitic acid makes up 36% of the total fatty acid. Extracts show no activity against E. faecalis, Y. enterolytica, E. aerogenes, B. cereus and E. agglomerans. Methanol extracts were active against S. enteritidis, S. cholerasius ser typhimurium and P. aeruginosa (minimum inhibitory concentration (MIC) 1 mg; zone of growth inhibition 7, 6.5 and 7 mm respectively). A queous extracts show activity against E. coli (MIC 5 mg) and P. aeruginosa (MIC 1 mg) while methanol extracts are active against P. aeruginosa and L. monocytogenes. G. latifolium has potential food and antibacterial uses.

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References

  • Ahamefule, F.O., Obua, B.E., Ibeawuchi, J.A., Udosen, N.R., 2006. The nutritive value of some plants browsed by cattle in Umudike, southeastern Nigeria. Pakistan J. Nutr., 5(5):404–409.

    Google Scholar 

  • Ajayi, I.A., Oderinde, R.A., Kajogbola, D.O., Ukponi, J.U., 2006. Oil content and fatty acid composition of some underutilized legumes from Nigeria. Food Chem., 99(1):115–120. [doi:10.1016/j.foodchem.2005.06.045]

    Article  CAS  Google Scholar 

  • Akwaowo, E.U., Ndon, B.A., Etuk, E.U., 2000. Minerals and antinutrients in fluted pumpkin (Telfairia ccidentalis Hook f.). Food Chem., 70(2):235–240. [doi:10.1016/S0308-8146(99)00207-1]

    Article  CAS  Google Scholar 

  • Aletor, V.A., Adeogun, O.A., 1995. Nutrient and anti-nutrient components of some tropical leafy vegetables. Food Chem., 53(4):375–379. [doi:10.1016/0308-8146(95)99830-S]

    Article  CAS  Google Scholar 

  • AOAC (Association of Official Analytical Chemist), 1990. Official Methods of Analysis, 15th Ed. Association of Official Analytical Chemists, Washington, DC.

    Google Scholar 

  • Apori, S.O., Long, R.J., Castro, F.B., Ørskov, E.R., 2000. Chemical composition and nutritive value of leaves and stems of tropical weed Chromolaena odorata. Grass and Forage Science, 55(1):77–81. [doi:10.1046/j.1365-2494.2000.00191.x]

    Article  Google Scholar 

  • Corlett, J.L., Clegg, M.S., Keen, C.L., Grivetti, L.E., 2002. Mineral content of culinary and medicinal plants cultivated by Hmong refugees living in Sacramento, California. Int. J. Food Sci. Nutr., 53(2):117–128. [doi:10.1080/09637480220132139]

    PubMed  Article  Google Scholar 

  • Eleyinmi, A.F., Bressler, D.C., Amoo, I.A., Sporns, P., Oshodi, A.A., 2006. Chemical composition of bitter cola (Garcinia kola) seed and hull. Polish J. Food Nutr. Sci., 15/56(4):27–32.

    Google Scholar 

  • Farombi, E.O., 2003. African indigenous plants with chemotherapeutic potentials and biotechnological approach to the production of bioactive prophylactic agents. Afr. J. Biotechnol., 2(12):662–671.

    Google Scholar 

  • Glew, R.H., VandeJagt, D.J., Lockett, C., Grivetti, L.E., Smith, G.C., Pastuszyn, A., Millson, M., 1997. Amino acid, fatty acid and mineral composition of 24 indigenous plants of Burkina Faso. J. Food Comp. Anal., 10(3):205–217. [doi:10.1006/jfca.1997.0539]

    Article  CAS  Google Scholar 

  • Glew, R.S., VanderJagt, D.J., Huang, Y.S., Chuang, L.T., Bosse, R., Glew, R.H., 2004. Nutritional analysis of the edible pit of Sclerocarya birrea in the Republic of Niger (daniya, Hausa). J. Food Comp. Anal., 17(1):99–111. [doi:10.1016/S0889-1575(03)00101-7]

    Article  CAS  Google Scholar 

  • Hernández, N.E., Tereschuk, M.L., Abdala, L.R., 2000. Antimicrobial activity of flavonoids in medicinal plants from Tafi del Valle (Tucuman, Argentina). J. Ethnopharmacol., 73(1–2):317–322. [doi:10.1016/S0378-8741(00)00295-6]

    PubMed  Article  Google Scholar 

  • Iqbal, A., Khalil, I.A., Ateeq, N., Khan, M.S., 2006. Nutritional quality of important food legumes. Food Chem., 97(2):331–335. [doi:10.1016/j.foodchem.2005.05.011]

    Article  CAS  Google Scholar 

  • Lauritzen, L., Hansen, H.S., Jorgensen, M.H., Michaelsen, K.F., 2001. The essentiality of long chain n-3 fatty acids in relation to development and function of the brain and retina. Prog. Lipid Res., 40(1–2):1–94. [doi:10.1016/S0163-7827(00)00017-5]

    PubMed  Article  CAS  Google Scholar 

  • Morebise, O., Fafunso, M.A., 1998. Antimicrobial and phytotoxic activities of saponin extracts from two Nigerian edible medicinal plants. Biokemistri, 8(2):69–77.

    CAS  Google Scholar 

  • Morebise, O., Fafunso, M.A., Makinde, J.M., Olajide, O.A., Awe, E.O., 2002. Antiinflamatory property of the leaves of Gongronema latifolium. Phytother. Res., 16(s1):S75–S77. [doi:10.1002/ptr.784]

    PubMed  Article  Google Scholar 

  • Ogundipe, O.O., Moody, J.O., Akinyemi, T.O., Raman, A., 2003. Hypoglycemic potentials of methanolic extracts of selected plant foods in alloxanized mice. Plant Foods Hum. Nutr., 58(3):1–7. [doi:10.1023/B:QUAL.0000040321.56831.c4]

    Article  Google Scholar 

  • Oshodi, A.A., Amoo, I.A., Eleyinmi, A.F., 2004. Antimicrobial activity of aqueous extracts of Vernonia amygdalina, Garcinia kola and Gongronema latifolium and their blends on some beer spoilage organisms. Technical Quarterly of the Master Brewers’ Association of the Americas, 41(4):398–402.

    Google Scholar 

  • Ross, I.A., 2001. Medicinal Plants of the World, Vol. 2: Chemical Constituents, Traditional and Modern Uses. Humane Press, Totowa NJ 07512, p.487.

    Google Scholar 

  • Schneider, C., Rotscheidt, K., Breitmaier, E., 1993. 4 new pregnane glycosides from Gongronema latifolium (Asckepiadaceae). Liebigs Annalen Der Chemie, 10:1057–1062.

    Google Scholar 

  • Sedgwick, G.W., Fenton, T.F., Thompson, J.R., 1991. Effect of protein precipitating agent on the recovery of plasma free amino acids. Can. J. Anim. Sci., 71:953–957.

    CAS  Article  Google Scholar 

  • Ugochukwu, N.H., Babady, N.E., 2002. Antioxidant effects of Gongronema latifolium in hepatocytes of rat models of non-insulin dependent diabetes mellitus. Fitoterapia, 73(7–8):612–618. [doi:10.1016/S0367-326X(02)00218-6]

    PubMed  Article  CAS  Google Scholar 

  • Ugochukwu, N.H., Babady, N.E., 2003. Antihyperglycemic effect of aqueous and ethanolic extracts of Gongronema latifolium leaves on glucose and glycogen metabolism in livers of normal and streptozotocin-induced diabetic rats. Life Sci., 73(15):1925–1938. [doi:10.1016/S0024-3205(03)00543-5]

    PubMed  Article  CAS  Google Scholar 

  • Ugochukwu, N.H., Babady, N.E., Cobourne, M., Gasset, S.R., 2003. The effect of Gongronema latifolium leaf extract on serum lipid profile and oxidative stress of hepatocytes of diabetic rats. J. Biosci., 28:1–5.

    PubMed  CAS  Article  Google Scholar 

  • Whiteside, P.J., Milner, B.A., 1984. Pye Unicam Atomic Absorption Data Book, 6th Ed. Pye Unicam Ltd., Cambridge, England, p.72.

    Google Scholar 

  • WHO, 1985. Energy and Protein Requirements. Report of a Joint FAO/WHO/UNU Expert Consultation. WHO Technical Report Series, No. 724.

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Eleyinmi, A.F. Chemical composition and antibacterial activity of Gongronema latifolium . J. Zhejiang Univ. - Sci. B 8, 352–358 (2007). https://doi.org/10.1631/jzus.2007.B0352

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  • DOI: https://doi.org/10.1631/jzus.2007.B0352

Key words

  • Gongronema latifolium
  • Chemical composition
  • Antibacterial activity

CLC number

  • S132