Skip to main content
Log in

First record of Bursaphelenchus rainulfi on pine trees from eastern China and its phylogenetic relationship with intro-genus species

  • Published:
Journal of Zhejiang University SCIENCE B Aims and scope Submit manuscript

Abstract

Bursaphelenchus rainulfi isolated from dead pine trees in Zhejiang, China, is described and illustrated. It also provided some molecular characters of the Chinese population, including the PCR-RFLP and sequences of ITS region and D2–D3 expansion region of the large subunit (LSU) rRNA gene. Both the morphological characters and ITS-RFLP patterns match with the original description. The phylogenetic trees based on the 13 sequences of D2–D3 expansion region of the LSU rRNA gene and ITS region of Bursaphelenchus species were constructed, respectively, with the results showing the similar clades. The phylogenetic relationship based on the molecular data is similar to that with morphological characters. This is the first report of the species on pine wood in eastern China.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Ambrogioni, L., Caroppo, S., 2002. II genere Bursaphelenchus in Europa Con Particolare Riferimento al complesso dei nematodi del legno del pino. Neumatologia Mediterranea, 30(Suppl.):95–106 (in Italian).

    Google Scholar 

  • Ambrogioni, L., Irdani, T., Caroppo, S., 2003. Records of Bursaphelenchus species on coniferous wood imported from Asian Russia and China to Italy. Reida, 86:139–146.

    Google Scholar 

  • Braasch, H., 2001. Brusaphelenchus species in conifers in Europe: distribution and morphological relationships. Bulletin OEPP/EPPO Bulletin, 31(2):127–142.

    Google Scholar 

  • Braasch, H., 2004. A new Bursaphelenchus species (Nematoda: Parasitaphelenchidae) sharing characters with Ektaphelenchidae from the People’s Republic of China. Zootaxa, 624:1–10.

    Google Scholar 

  • Braasch, H., Burgermeister, W., 2002. Bursaphelenchus rainulfi sp. n. (Nematoda: Parasitaphelenchidae), first record of the genus Bursaphelenchus Fuchs, 1937 from Malaysia. Nematology, 4(8):971–978. [doi:10.1163/156854102321122593]

    Article  Google Scholar 

  • Braasch, H., Gu, J.F., Burgermeister, W., Zhang, J.C., 2005. Bursaphelenchus doui sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Taiwan and South Korea—a new species of the xylophilus group. Russian Journal of Nematology, 13(1):19–27.

    Google Scholar 

  • Burgermeister, W., Metge, K., Braasch, H., Buchbach, E., 2005. ITS-RFLP patterns for differentiation of 26 Bursaphelenchus species (Nematoda: Parasitaphelenchidae) and observations on their distribution. Russian Journal of Nematology, 13(1):29–42.

    Google Scholar 

  • Dan, Y., Yang, P.W., Li, J.L., Yu, S.F., 2003. PCR amplification of ribosomal DNA in four species of Bursaphelenchus. Journal of Yunnan Agricultural University, 18(3):309–311 (in Chinese).

    CAS  Google Scholar 

  • de Ley, P., Félix, M.A., Frisse, L.M., Nadler, S.A., Sternberg, P.W., Thomas, W.K., 1999. Molecular and morphological characterization of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology, 1(6):591–612. [doi:10.1163/156854199508559]

    Article  Google Scholar 

  • Evans, H.F., Mcnamara, D.G., Braasch, H., Chadoeuf, J., Magnusson, C., 1996. Pest risk analysis (PRA) for the territories of the European Union (as PRA area) on Bursaphelenchus xylophilus and its vectors in the genus Monochamus. OEPP/EPPO Bulletin, 26(2):199–249.

    Google Scholar 

  • Fang, Y.S., Zhao, J., Zhuo, K., 2002a. Description of Bursaphelenchus dongguanensis sp. n. in China (Nematoda: Aphelenchoididae). Journal of Huazhong Agricultural University, 21(2):109–111 (in Chinese).

    Google Scholar 

  • Fang, Y.S., Zhuo, K., Zhao, J., 2002b. Description of Bursaphelenchus aberrans n. sp. (Nematoda: Parasitaphelenchidae) isolated from pine wood in Guangdong Province, China. Nematology, 4(7):791–794. [doi:10.1163/1568541027600402577]

    Article  Google Scholar 

  • Ferris, V.R., Ferris, J.M., Faghihi, J., 1993. Variations in spacer ribosomal DNA in some cyst forming species of plant parasitic nematodes. Fundamental and Applied Nematology, 16(2):177–184.

    Google Scholar 

  • He, Y., Subbotin, S., Rubtsova, T.V., Lamberti, F., Brown, D.J.F., Moens, M., 2005. A molecular phylogenetic approach to Longidoridae (Nematoda: Dorylaimida). Nematology, 7(1):111–124. [doi:10.1163/1568541054192108]

    Article  CAS  Google Scholar 

  • Hoyer, V.U., Burgermeister, W., Braasch, H., 1998. Identification of Bursaphelenchus species (Nematoda: Aphelenchoididae) on the basis of amplified ribosomal DNA (ITS-RFLP). Nachrichtenblatt des Deutschen Pflanzenschutzdiensites, 50(11):273–277.

    Google Scholar 

  • Hunt, D.J., 1993. Aphelenchida, Longidoridae and Trichodoridae, Their Systematics and Bionomics. Cambridge University Press, UK, p.106–109.

    Google Scholar 

  • Iwahori, H., Tsuda, K., Kanzaki, N., Izui, K., Ftai, K., 1998. PCR-RFLP and sequencing analysis of ribosomal DNA of Bursaphelenchus nematodes related to pine wilt disease. Fundamental and Applied Nematology, 21(6):655–666.

    CAS  Google Scholar 

  • Maafi, Z.T., Subbotin, S.A., Moens, M., 2003. Molecular identification of cyst-forming nematodes (Heteroderidae) from Iran and a phylogeny based on ITS-rDNA sequences. Nematology, 5(1):99–111. [doi:10.1163/156854102765216731]

    Article  CAS  Google Scholar 

  • Madani, M., Vovlas, N., Castillo, P., Subbotin, S.A., Moens, M., 2004. Molecular characterization of cyst nematode species (Heterodera spp.) from the Mediterranean Basin using RFLPs and sequences of ITS-rDNA. Journal of Phytopathology, 152(4):229–234. [doi:10.1111/j.1439-0434.2004.00835.x]

    Article  CAS  Google Scholar 

  • Mamiya, Y., Enda, N., 1979. Bursaphelenchus mucronatus n. sp. (Nematoda: Aphelenchoididae) from pine wood and its biology and pathogenicity to pine trees. Nematologica, 25(3):353–361.

    Article  Google Scholar 

  • Palmisano, A.M., Ambrogioni, L., Tomiczek, C., Brandstetter, M., 2004. Bursaphelenchus sinensis sp. n. and B. thailandae Braasch et Braasch-Bidasak in Packaging wood from China. Nematologia Mediterranea, 32(1):57–65 (in Italian).

    Google Scholar 

  • Ryss, A., Vieira, P., Mota, M., Kulinich, O., 2005. A synopsis of the genus Bursaphelenchus Fuchs, 1937 (Aphelenchida: Parasitaphelenchidae) with key to species. Nematology, 7(3):393–458. [doi:10.1163/156854105774355581]

    Article  Google Scholar 

  • Subbotin, S.A., Sturhan, D., 2004. Heterodera circeae sp. n. and H. scutellariae sp. n. (Tylenchida: Heteroderidae) from Germany, with notes on the goettingiana group. Nematology, 6(3):343–355. [doi:10.1163/1568541042360582]

    Article  Google Scholar 

  • Vrain, T.C., 1993. Restriction fragment length polymorphism separates species of Xiphinema americanum group. Journal of Nematology, 25(3):361–364.

    CAS  PubMed  Google Scholar 

  • Wang, H., Pan, C., Chen, Y., 2004. A new record species of genus Bursaphelenchus Fuchs, 1937 (Nematoda: Parasitaphelenchidae) in Pinus massoniana from China. Journal of Xiamen University, 5(5):727–732 (in Chinese).

    Google Scholar 

  • Yin, G.L., Fang, Y.S., 1995. The new record of Bursaphelenchus from China (Aphelenchida: Parasitaphelenchidae). Act5a Zootaxonomica Sinica, 20(1):5.

    Google Scholar 

  • Yin, K.C., Fang, Y.S., Tarjan, A.C., 1988. The key to species in the genus Bursaphelenchus with a description of Bursaphelenchus hunanensis sp. n. (Nematoda: Aphelenchoididae) found in pine wood in Hunan Province China. Proceedings of the Helminthological Society of Washington, 55(1):1–11.

    Google Scholar 

  • Zheng, J., Subbotin, S., He, S., Gu, J., Moens, M., 2003. Molecular characterization of some Asian isolates of Bursaphelenchus xylophilus and B. mucronatus using PCR-RFLPs and sequences of ribosomal DNA. Russian Journal of Nematology, 11(1):17–22.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Zheng Jing-wu.

Additional information

Project (No. 2002C34003) supported by the Science and Technology Department of Zhejiang Province, China

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jiang, Lq., Li, Xq. & Zheng, Jw. First record of Bursaphelenchus rainulfi on pine trees from eastern China and its phylogenetic relationship with intro-genus species. J. Zhejiang Univ. - Sci. B 8, 345–351 (2007). https://doi.org/10.1631/jzus.2007.B0345

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1631/jzus.2007.B0345

Key words

CLC number

Navigation