Abstract
The use of periosteum-derived progenitor cells (PCs) combined with bioresorbable materials is an attractive approach for tissue engineering. The aim of this study was to characterize the osteogenic differentiation of PC in 3-dimensional (3D) poly-lactic-co-glycolic acid (PLGA) fleeces cultured in medium containing allogeneic human serum. PCs were isolated and expanded in monolayer culture. Expanded cells of passage 3 were seeded into PLGA constructs and cultured in osteogenic medium for a maximum period of 28 d. Morphological, histological and cell viability analyses of three-dimensionally cultured PCs were performed to elucidate osseous synthesis and deposition of a calcified matrix. Furthermore, the mRNA expression of type I collagen, osteocalcin and osteonectin was semi-quantitively evaluated by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). The fibrin gel immobilization technique provided homogeneous PCs distribution in 3D PLGA constructs. Live-dead staining indicated a high viability rate of PCs inside the PLGA scaffolds. Secreted nodules of neo-bone tissue formation and the presence of matrix mineralization were confirmed by positive von Kossa staining. The osteogenic differentiation of PCs was further demonstrated by the detection of type I collagen, osteocalcin and osteonectin gene expression. The results of this study support the concept that this tissue engineering method presents a promising method for creation of new bone in vivo.
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References
Arnold, U., Lindenhayn, K., Perka, C., 2002. In vitro-cultivation of human periosteum derived cells in bioresorbable polymer-TCP-composites. Biomaterials, 23(11):2303–2310. [doi:10.1016/S0142-9612(01)00364-7]
Barry, F.P., Murphy, J.M., 2004. Mesenchymal stem cells: clinical applications and biological characterization. Int. J. Biochem. Cell Biol., 36(4):568–584. [doi:10.1016/j.biocel.2003.11.001]
Brittberg, M., Lindahl, A., Nilsson, A., Ohlsson, C., Isaksson, O., Peterson, L., 1994. Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N. Engl. J. Med., 331(14):889–895. [doi:10.1056/NEJM199410063311401]
Chen, G., Sato, T., Ohgushi, H., Ushida, T., Tateishi, T., Tanaka, J., 2005. Culturing of skin fibroblasts in a thin PLGA-collagen hybrid mesh. Biomaterials, 26(15):2559–2566. [doi:10.1016/j.biomaterials.2004.07.034]
Chenu, C., Colucci, S., Grano, M., Zigrino, P., Barattolo, R., Zambonin, G., Baldini, N., Vergnaud, P., Delmas, P.D., Zallone, A.Z., 1994. Osteocalcin induces chemotaxis, secretion of matrix proteins, and calcium-mediated intracellular signaling in human osteoclast-like cells. J. Cell Biol., 127(4):1149–1158. [doi:10.1083/jcb.127.4.1149]
de Bari, C., Dell’Accio, F., Tylzanowski, P., Luyten, F.P., 2001. Multipotent mesenchymal stem cells from adult human synovial membrane. Arthritis Rheum., 44(8):1928–1942. [doi:10.1002/1529-0131(200108)44:8〈1928::AID-ART331〉3.0.CO;2-P]
Derubeis, A.R., Cancedda, R., 2004. Bone marrow stromal cells (BMSCs) in bone engineering: limitations and recent advances. Annals of Biomedical Engineering, 32(1):160–165. [doi:10.1023/B:ABME.0000007800.89194.95]
Gröger, A., Klaring, S., Merten, H.A., Holste, J., Kaps, C., Sittinger, M., 2003. Tissue engineering of bone for mandibular augmentation in immunocompetent minipigs: preliminary study. Scand. J. Plast. Reconstr. Surg. Hand Surg., 37(3):129–133. [doi:10.1080/02844310310007728]
Ignatius, A., Blessing, H., Liedert, A., Schmidt, C., Neidlinger-Wilke, C., Kaspar, D., Friemert, B., Claes, L., 2005. Tissue engineering of bone: effects of mechanical strain on osteoblastic cells in type I collagen matrices. Biomaterials, 26(3):311–318. [doi:10.1016/j.biomaterials.2004.02.045]
Karp, J.M., Sarraf, F., Shoichet, M.S., Davies, J.E., 2004. Fibrin-filled scaffolds for bone-tissue engineering: an in vivo study. J. Biomed. Mater. Res. A, 71(1):162–171. [doi:10.1002/jbm.a.30147]
Lennon, P.F., Collard, C.D., Morrissey, M.A., Stahl, G.L., 1996. Complement-induced endothelial dysfunction in rabbits: mechanisms, recovery, and gender differences. Am. J. Physiol., 270(6 Pt 2):H1924–H1932.
Muschler, G.F., Midura, R.J., 2002. Connective tissue progenitors: practical concepts for clinical applications. Clin. Orthop. Relat. Res., 395:66–80. [doi:10.1097/00003086-200202000-00008]
Nöth, U., Osyczka, A.M., Tuli, R., Hickok, N.J., Danielson, K.G., Tuan, R.S., 2002. Multilineage mesenchymal differentiation potential of human trabecular bone-derived cells. J. Orthop. Res., 20(5):1060–1069. [doi:10.1016/S0736-0266(02)00018-9]
Ouyang, H.W., Goh, J.C., Mo, X.M., Teoh, S.H., Lee, E.H., 2002. The efficacy of bone marrow stromal cell-seeded knitted PLGA fiber scaffold for Achilles tendon repair. Ann. N.Y. Acad. Sci., 961(1):126–129.
Peng, H., Huard, J., 2004. Muscle-derived stem cells for musculoskeletal tissue regeneration and repair. Transpl. Immunol., 12(3–4):311–319. [doi:10.1016/j.trim.2003.12.009]
Perka, C., Schultz, O., Spitzer, R.S., Lindenhayn, K., Burmester, G.R., Sittinger, M., 2000. Segmental bone repair by tissue-engineered periosteal cell transplants with bioresorbable fleece and fibrin scaffolds in rabbits. Biomaterials, 21(11):1145–1153. [doi:10.1016/S0142-9612(99)00280-X]
Pittenger, M.F., Mackay, A.M., Beck, S.C., Jaiswal, R.K., Douglas, R., Mosca, J.D., Moorman, M.A., Simonetti, D.W., Craig, S., Marshak, D.R., 1999. Multilineage potential of adult human mesenchymal stem cells. Science, 284(5411):143–147. [doi:10.1126/science.284.5411.143]
Redlich, A., Perka, C., Schultz, O., Spitzer, R., Häupl, T., Burmester, G.R., Sittinger, M., 1999. Bone engineering on the basis of periosteal cells cultured in polymer fleeces. J. Mater. Sci. Mater. Med., 10(12):767–772. [doi:10.1023/A:1008994715605]
Ringe, J., Kaps, C., Burmester, G.R., Sittinger, M., 2002. Stem cells for regenerative medicine: advances in the engineering of tissues and organs. Naturwissenschaften, 89(8):338–351. [doi:10.1007/s00114-002-0344-9]
Ringe, J., Zheng, Y.X., Neumann, K., 2005. Surface marker expression, multilinage potential and chemotaxis of human mesenchymal stem cell and periosteal cells. The International Journal of Artificial Organ, 28(4):336.
Schmelzeisen, R., Schimming, R., Sittinger, M., 2003. Making bone: implant insertion into tissue-engineered bone for maxillary sinus floor augmentation—a preliminary report. J. Craniomaxillofac. Surg., 31(1):34–39.
Sittinger, M., Reitzel, D., Dauner, M., Hierlemann, H., Hammer, C., Kastenbauer, E., Planck, H., Burmester, G.R., Bujia, J., 1996. Resorbable polyesters in cartilage engineering: affinity and biocompatibility of polymer fiber structures to chondrocytes. J. Biomed. Mater. Res., 33(2):57–63. [doi:10.1002/(SICI)1097-4636(199622)33:2〈57::AID-JBM1〉3.0.CO;2-K]
Sittinger, M., Hutmacher, D.M., Risbud, M.V., 2004. Current strategies for cell delivery in cartilage and bone regeneration. Curr. Opin. Biotechnol., 15(5):411–418. [doi:10.1016/j.copbio.2004.08.010]
Sommer, B., Bickel, M., Hofstetter, W., Wetterwald, A., 1996. Expression of matrix proteins during the development of mineralized tissues. Bone, 19(4):371–380. [doi:10.1016/S8756-3282(96)00218-9]
Zuk, P.A., Zhu, M., Ashjian, P., de Ugarte, D.A., Huang, J.I., Mizuno, H., Alfonso, Z.C., Fraser, J.K., Benhaim, P., Hedrick, M.H., 2002. Human adipose tissue is a source of multipotent stem cells. Mol. Biol. Cell, 13(12):4279–4295. [doi:10.1091/mbc.E02-02-0105]
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Project supported by the Investitionsbank Berlin (IBB), Germany (No. 10020666) and the Science and Technology Bureau of Zhejiang Province, China (No. 991110052)
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Zheng, Yx., Ringe, J., Liang, Z. et al. Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum. J. Zhejiang Univ. - Sci. B 7, 817–824 (2006). https://doi.org/10.1631/jzus.2006.B0817
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DOI: https://doi.org/10.1631/jzus.2006.B0817
Key words
- Tissue engineering
- Poly-lactic-co-glycolic acid polymer
- Periosteum-derived progenitor cells
- 3-dimensional culture