Abstract
Wild cotton species can contribute a valuable gene pool for agronomically desirable cultivated tetraploid cultivars. In order to exploit diploid cotton a regeneration system is required to achieve transformation based goals. The present studies aimed at optimizing the conditions for regeneration of local varieties as well as wild species of cotton. Different callus induction media were tested with varying concentrations of hormones in which sucrose was used as nutritional source. Different explants (hypocotyls, cotyledon, root) were used to check the regeneration of both local cotton plants and wild relatives using T & G medium, BAP medium, CIM medium, EMMS medium, and cell suspension medium. Different stages of embryogenicity such as early torpedo stage, late torpedo stage, heart stage, globular stage and cotyledonary stage were observed in wild relatives of cotton. The results of this study pave the way for establishing future transformation methods.
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References
Chee, R.P., Schultheis, J.R., Cantliffe, D.J., 1990. Plant recovery from sweet potato somatic embryos. Hortscience, 25:795–797.
Evans, D.A., Sharp, W.R., 1981. Growth and Behavior of Cell Cultures: Embryogenesis and Organogenesis. In: Trevor, A.T. (Ed.), Plant Tissue Cultures. Academic Press, New York, p.45–113.
Finer, J.J., 1988. Plant regeneration from somatic embryogenesis suspension culture of cotton (Gossypium hirsutum L.). Plant Cell Rep., 7:399–402.
Finer, J.J., Smith, R.H., 1984. Initiation of callus and somatic embryos from explants of mature cotton (Gossypium Klotzschianum Andress L.). Plant Cell Rep., 3(1):41–43. [doi:10.1007/BF00270228]
Firoozabady, E., DeBoer, D.L., 1993. Plant regeneration via somatic embryogenesis in many cultivars of cotton (Gossypium hirstum L.). In vitro Cell. Dev. Biol. Plant, 29:166–173.
Fredrick, J.R., Randy, W.D., Tony, A.A., Roy, L.F., Stevin, R.S., Johnson, T., GandDavis, A.F., 1990. Insect resistant cotton plant. Biotechnology, 11:1151–1156.
Gayle, H.D., Hamilton, H.R., 1983. Plant regeneration from callus tissue of (Gossypium hirsutum L.). Plant Science Letters, 3:89–93.
Imin, N., Nizamidin, M., Daniher, D., Nolan, K.E., Rose, R.J., Rolfe, B.G., 2005. Proteomic analysis of somatic embryogenesis in Medicago truncatula. Explant cultures grown under 6-benzylaminopurine and 1-naphthaleneacetic acid treatments. Plant Physiol., 137(4):1250–1260. [doi:10.1104/pp.104.055277]
Lazzeri, P.A., Hildebrand, D.F., Collins, G.B., 1987. Soybean somatic embryogenesis: effect of nutritional, physical and chemical factors. Plant Cell, Tissue and Organ Culture, 10(3):209–220. [doi:10.1007/BF00037305]
Pérez, C., Fernández, B., Rodríguez, R., 1983. In vitro plantlet regeneration through asexual embryogenesis in cotyledonary segments of Corylus avellana L. Plant Cell Rep., 2(5):226–228. [doi:10.1007/BF00269146]
Price, H.J., Smith, R.H., 1979. Somatic embryogenesis in suspension cultures Gossypium klotzschienum Andress. Planta, 145(3):305–307. [doi:10.1007/BF00454456]
Rajasekaran, K., Grula, J.W., Hudspeth, R.L., Pofelis, S., Anderson, D.M., 1996. Herbicide resistant Acala and Coker transformed with a native gene encoding mutant forms of acetohydroxy acid synthase. Molecular Breeding, 2(4):307–319. [doi:10.1007/BF00437909]
Sakhanokho, H., Sharma, G.C., Zipf, A., Saha, S., Rajasekaran, K., 1999. Tissue Culture Potential of Diverse Diploid and Tetraploid Cotton Genotypes. Proc. Beltwide Cotton Conf., San Diego, CA, p.590–592.
Sakhanokho, H.F., Zip, F.A., Rajasekaran, K., Saha, S., Sharma, G.C., 2001. Induction of highly embryogenic calli and plant regeneration in upland (Gossypium hirsutum L.) and Pima (Gossypium barbadense L.) cottons. Crop Science, 41:1235–1240.
Scowcroft, W.R., 1984. Tech. Rep. Internative Board for Plant Gen. Res. (AGPG:IBPGR), p.41.
Shoemaker, R.C., Couche, L.J., Galbraith, D.W., 1986. Characterization of somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.). Plant Cell Rep., 5(3):178–181. [doi:10.1007/BF00269112]
Stamp, J.A., Henshaw, G.G., 1982. Somatic embryogenesis in cassava. Z Pflanzenphysiol, 105:183–187.
Trolinder, N.L., Xhixian, C., 1989. Genotype specificity of the somatic embryogenesis response in cotton. Plant Cell Rep., 8(3):133–136 [doi:10.1007/BF00716824]
Zhang, B.H., Feng, R., Li, F.L., Li, X.L., 1996. Anther culture and plant regeneration of cotton (Gossypium klotzchianum Anderss). Chinese Science Bulletin, 41:145–148.
Zhang, B.H., Liu, F., Yao, C.B., Wang, K.B., 2000. Plant regeneration via somatic embryogensis in cotton. Plant Cell, Tissue and Organ Culture, 60(2):89–94. [doi:10.1023/A:1006488119200]
Zhang, B.H., Wang, L.Q., Liu, F., 2001. Phenotypic variation in cotton (Gossypium hirsutum L.) regenerated plants. Current Science, 81:1112–1115.
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Rao, A.Q., Hussain, S.S., Shahzad, M.S. et al. Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.). J. Zhejiang Univ. - Sci. B 7, 291–298 (2006). https://doi.org/10.1631/jzus.2006.B0291
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DOI: https://doi.org/10.1631/jzus.2006.B0291