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Automatic separation system for marine meiobenthos based on laser-induced fluorescence technology

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Abstract

An automatic system for marine meiobenthos separation was developed by using laser-induced fluorescence technology. Rose Bengal was used as organism dye and the spectrums of Rose Bengal were measured. Laser-induced fluorescence system was established to detect marine meiobenthos in sediments. Data obtained from experiments were analyzed by using a mathematical model. The results showed that laser-induced fluorescence technology worked well in the system. The system could select the meiobenthos efficiently and precisely.

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References

  • Chuck, R.S., Arnoldussen, M.E., Behrens, A., 2002. Fluorescence-guided laser removal of chemicaly damaged cornea.Journal of Cataract & Refractive Surgery,28(10):1847–1852.

    Article  Google Scholar 

  • De Jonge, V.N., Bouwman, L.A., 1977. A simple density separation technique for quantitative isolation of meiobenthos using the colloidal silica Ludox-TM.Marine Biology,42(2):143–148.

    Article  Google Scholar 

  • Dillon, W.P., 1964. Flotation technique for separating fecal pellets and small marine organisms from scand.Limnology and oceanography,9:601–602.

    Article  Google Scholar 

  • Gwyther, D., 2004. Impact of the deposition of jarosite on deepwater meiobenthos in southeastern.Australia Marine Pollution Bulletin,48(9–10):997–1003.

    Article  CAS  Google Scholar 

  • Heip, C., Smol, N., Hautekiet, W., 1974. A rapid method for extracting meiobenthic Nematodes and Copepods from mud and detritus.Marine Biology,28:79–81.

    Article  Google Scholar 

  • Jensen, P., 1982. A new meiofauna sample splitter.Annales Zoologici Fennici,19:233–236.

    Google Scholar 

  • Kitazato, H., Nakatsuka, T., Shimanaga, M., Kanda, J., Soh, W., Kato, Y., Okada, Y., Yamaoka, A., Masuzawa, T., Suzuki, K., Shirayama, Y., 2003. Long-term monitoring of the sedimentary processes in the central part of Sagami Bay, Japan: Rationale, logistics and overview of results.Progress In Oceanography,57(1):3–16.

    Article  Google Scholar 

  • Kopp, M.U., Crabtree, H.J., Manz, A., 1997. Developments in technology and applications of Microsystems.Current Opinion in Chemical Biology,1(3):410–419.

    Article  CAS  Google Scholar 

  • Larkin, J.M., Donaldson, W.R., Foster, T.H., Knox, R.S., 1999. Reverse intersystem crossing from a triplet state of rose Bengal populated by sequential 532 nm plus 1064 nm laser excitation.Chemical Physics,244:319–330.

    Article  CAS  Google Scholar 

  • Li, Y.B., Zhu, J., Yang, Y.L., Xu, J., Zhang, H.G., Shi, H.M., 1996. The establishment of laser-induce fluorescence detection system.Laser Medicine,6(3):97–102.

    CAS  Google Scholar 

  • Lu, X., Huang, W.H., Wang, Z.L., Cheng, J.K., 2004. Recent developments in single-cell analysis.Analytica Chimica Acta,510(2):127–138.

    Article  CAS  Google Scholar 

  • Mclntyre, A.D., 1969. Ecology of marine meiobenthos.Biological Reviews,44:245–290.

    Article  Google Scholar 

  • Michael, A., Daniel, R., Armstrong, W., 2004. Separation and analysis of colloidal/nano-particles including microoranisms by capillary electrophoresis: A fundamental review.Journal of Chromatography B,800(1–2):7–25.

    Google Scholar 

  • Nichols, J.A., 1979. A simple flotation technique for separation meiobenthic nematodes from fine-grained sediments.Trasactions of American Microscopical Society,98: 127–130.

    Article  Google Scholar 

  • Schwinghamer, P., 1981. Extraction of living meiofauna from marine sediments by centrifugation in a silica Sol-Sorbitol mixture.Canadian Journal of Fisheries and Aquatic Sciences,38:476–478.

    Article  Google Scholar 

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Project (Grant No. DY105-03-01-15) supported by “Tenth Five Year Plan of R & D of International Deep-Sea” of National Ocean Mineral Resources R & D Association, China

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Ye, Sm., Gao, Sj., Pan, Y. et al. Automatic separation system for marine meiobenthos based on laser-induced fluorescence technology. J Zheijang Univ Sci B 6, 535–539 (2005). https://doi.org/10.1631/jzus.2005.B0535

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  • DOI: https://doi.org/10.1631/jzus.2005.B0535

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