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Cytotoxicity of epigallocatechin-3-gallate to LNCaP cells in the presence of Cu2+

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Abstract

Epigallocatechin-3-gallate (EGCG) has shown remarkably anti-cancer activity, with its bioactivity being related to reactive conditions such as pH and metal ions. The present study investigated the degradation of EGCG and its effect on prostate cancer cell in the presence of Cu2+. EGCG was incubated with prostate cancer cells, LNCaP, pretreated with or without Cu2+. EGCG in F-12 medium was quantified using HPLC and the viability of cells was assessed by gel electrophoresis, flow cytometry, and electron microscope. The results of HPLC showed that EGCG degraded completely within 12 h in F-12 medium with or without Cu2+. Gel electrophoresis and flow cytometry did not detect apoptosis of LNCaP cells when they were incubated with EGCG. Electron microscopy examination revealed that EGCG-Cu2+ complex led to damage of cytoplasm membrane in LNCaP cells. It was speculated that not EGCG, but its oxide and complex with Cu2+, are the bioactive components responsible for its cytotoxicity to LNCaP prostate cancer cells.

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Authors and Affiliations

  1. Department of Tea Science, School of Agriculture and Biotechnology, Zhejiang University, 310009, Hangzhou, China

    Hai-ning Yu & Sheng-rong Shen

  2. Department of Pharmacology, Zhejiang College of Traditional Chinese Medicine, 310009, Hangzhou, China

    Yao-kang Xiong

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  1. Hai-ning Yu
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  2. Sheng-rong Shen
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  3. Yao-kang Xiong
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Correspondence to Sheng-rong Shen.

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Project (No. 3047198) supported by the National Natural Science Foundation of China

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Yu, Hn., Shen, Sr. & Xiong, Yk. Cytotoxicity of epigallocatechin-3-gallate to LNCaP cells in the presence of Cu2+. J Zheijang Univ Sci B 6, 125–131 (2005). https://doi.org/10.1631/jzus.2005.B0125

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  • DOI: https://doi.org/10.1631/jzus.2005.B0125

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