Abstract
Objective: To construct a eukaryotic expression plasmidpcDNA3.1(−)-Humanin. Methods: The recombinant plasmid pGEMEX-1-Humanin was digested with restriction endonucleases BamH I and Hind III and the Humanin gene fragments, about 100 bp length, were obtained. Then the Humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1(−) and the recombinant plasmids pcDNA3.1(−)-Humanin were identified by sequencing. Results: Recombinant plasmid DNA successfully produced a band which had the same size as that of the Humanin positive control. The sequence of recombinant plasmids accorded with the Humnain gene sequence. Conclusions: A eukaryotic expression plasmid of Humanin was successfully constructed.
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Hashimoto, Y., Ito, Y., Niikura, T., Shao, Z., Hata, M., Oyama, F., Nishimoto, I.,2001a. Mechanisms of neuroprotection by a novel rescue factor Humanin from Swedish mutant amyloid precursor protein.Biochem Biophys Res Commun,283(2):460–468.
Hashimoto, Y., Niikura, T., Ito, Y., Sudo, H., Hata, M., Arakawa, E., Abe, Y., Kita, Y., Nishimoto, I., 2001b. Detailed characterization of neuroprotection by a rescue factor HN against various Alzheimer’s disease-relevant insults.J Neurosci,21(23):235–9245.
Hashimoto, Y., Niikura, T., Tajima, H., Yasukawa, T., Sudo, H., Ito, Y., Kita, Y., Kawasumi, M., Kouyama, K., Doyu, M., Sobue, G., Koide, T., Tsuji, S., Lang, J., Kurokawa, K., Nishimoto, I., 2001c. A rescue factor abolishing neuronal cell death by a wide spectrum of familial Alzheimer’s disease genes and Abeta.Proc Natl Acad Sci USA,98(11):6336–6341.
Luo, B.Y., Yuan, M., Tang, M., Chen, Z., 2004. Cloning and expression of the anti-AD protective peptide Humanin.Chin J Neurology,37(3):231–233 (in Chinese).
Niikura, T., Hashimoto, Y., Tajima, H., Nishimoto, I., 2002. Death and survival of neuronal cells exposed to Alzheimer’s insults.J Neurosci Res,70(3):380–391.
Terashita, K., Hashimoto, Y., Niikura, T., Tajima, H., Yamagishi, Y., Ishizaka, M., Kawasumi, M., Chiba, T., Kanekura, K., Yamada, M., Nawa, M., Kita, Y., Aiso, S., Nishimoto, I., 2003. Two serine residues distinctly regulate the rescue function of Humanin, an inhibiting factor of Alzheimer’s disease-related neurotoxicity: functional potentiation by isomerization and dimerization.J Neurochem,85(6): 1521–1538.
Zou, P., Ding, Y., Sha, Y., Hu, B., Nie, S., 2003. Humanin peptides block calcium influx of rat hippocampal neurons by altering fibrogenesis of Abeta (1–40).Peptides,24(5):679–685.
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Project (No. 2003C30040) supported by the Science and Technology Department of Zhejiang Province, China
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Luo, By., Chen, Xm., Tang, M. et al. Construction of a eukaryotic expression plasmid of Humanin. J Zheijang Univ Sci B 6, 11–13 (2005). https://doi.org/10.1631/jzus.2005.B0011
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DOI: https://doi.org/10.1631/jzus.2005.B0011