Abstract
The cucumber mosaic virus (CMV) isolate P1 caused very mild symptoms on many plant species After serial passages by mechanical inoculation over five years, CMV P1 caused severe symptoms on several tobacco cultivars and tomato. A specific band of approximately 0.3 kb in length was amplified by RT-PCR with primers synthesized based on reported CMV satellite RNA (satRNA) sequences. Sequence analysis showed there were two satRNAs (Sat-P1-1 and Sat-P1-2). Sat-P1-1 contained 335 nucleotides, and Sat-P1-2 contained 394 nucleotides. These two satRNAs shared 64% overall nucleotide sequence homology, and differences between the two satRNAs included mutations as well as deletions. Sat-P1-1 was identical to a satRNA (Z96099) reported in 1995 in CMV P1. Based on differences in the sequence and secondary structure between these two satRNAs, we conclude that Sat-P1-2 represents the emergence of a new satellite (necrotic satellite) from attenuated satRNA populations. The possible effect of the emergence of this new satRNA is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cheng, N. H., Fang, R. X., Pu, Z. Q. and Fang, Z. D., 1997. Complete nucleotide sequence of a new satellite RNA associated with cucumber mosaic virus inducing tomato necrosis.Science in China Series C,40: 443–448.
Garcia-Arenal, F., Escriu, F., Aranda, M. A., Alonso-Prados, J. L., Malpica, J. M. and Fraile, A., 2000. Molecular epidemiology of cucumber mosaic virus and its satellite RNA.Virus Research,71: 1–8.
Grieco, F., Lanave, C. and Gallitelli, D., 1997. Evolutionary dynamics of cucumber mosaic virus satellite RNA during natural epidemics in Italy.Virology,229: 166–174.
Tien, P. and Wu, G., 1991. Satellite RNA for the biocontrol of plant disease.Adv. Virus Res,39: 321–339.
Zhou, X., Pu, Z. Q. and Fang, Z. D., 1994. Isolation of a mild strain of CMV with satellite RNA and cross protection against virulent strains.Virology Sinica,9: 319–326.
Zhou, X., Liu, Y., Xue, C. Y. and Li, D. B., 1995b. Cloning and sequence analysis of satellite RNA of cucumber mosaic virus mild strain CMV P1.Journal of Zhejiang Agricultural University,21: 563.
Zhou, X. P., Xu, Z. X., Xu, J. and Li, D. B., 1995b. Study on cucumber mosaic virus infectingLuffer cylidrica.Journal of South China Agriculture University,16 (2): 74–79.
Zhou, X. P., Xue, C. Y., Chen, Q., Qi, Y. J. and Li, D. B., 2000. Complete nucleotide sequence and genome organization of tobacco mosaic virus isolated fromVicia faba.Science in China Series C,43: 200–208.
Author information
Authors and Affiliations
Corresponding author
Additional information
Project supported by research grants from the Teaching and Research Award Program for Outstanding Teachers in Higher Education Institutes of the Ministry of Education of China and by the National Outstanding Youth Foundations of National Natural Science Foundation of China (No. 30125032).
PhD student in Zhejiang University, from Agrarian University of Havana, Cuba.
Rights and permissions
About this article
Cite this article
Alvarez, S.P., Xue, Cy. & Zhou, Xp. Emergence of a new satellite RNA from cucumber mosaic virus isolate P1. J. Zhejiang Univ. Sci. A 4, 336–339 (2003). https://doi.org/10.1631/jzus.2003.0336
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1631/jzus.2003.0336