Abstract
This paper presents a feasible method for rapid detection of the interphase nuclei of uncultured amniocytes for chromosomes 18 by using our modified primed in situ labeling (PRINS) technique. A total of 262 independent, uncultured amniotic fluid samples were analyzed in a blind fashion before the karyotype was available. In addition, 62 samples were examined by fluorescence in situ hybridization (FISH) for comparison. In more than 95% of the samples PRINS reactions with prime 18cen were successfully induced. Two samples were properly identified and correctly soored as trisomic 18. PRINS reaction could be performed automatically in less than one hour with a programmable themocycler. Our studies showed that the PRINS technique is simple, rapid and cost-effective. It is as sensitive and specific as FISH; can enhance the accuracy of standard cytogenetic analysis; and allows identification of chromosomes 18 aneuploidies in uncultured amniocytes in significantly less time.
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Project supported by the Scientific Research Foundation for Returned Overseas Chinese Scholars, State Education Ministry and Postdoctoral Research Foundation of China
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Jian-bin, Y., Shu, Z. Rapid detection of chromosome 18 aneuploidies in amniocytes by using Primed In Situ Labeling (PRINS) technique. J. Zhejiang Univ. Sci. A 3, 617–621 (2002). https://doi.org/10.1631/jzus.2002.0617
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DOI: https://doi.org/10.1631/jzus.2002.0617