Abstract
Genome segments S8 of two Chinese isolates of rice black-streaked dwarf virus (RBSDV), one from Zhejiang Province and another from Hebei Province, were amplified by RT-PCR and sequenced. Both segments consisted of 1936 nts in full length (EMBL accession numbers were AJ297431 and AJ297432, respectively) and contained only one big open reading frame which encoded a polypeptide with molecular weight of 68kD. The two Chinese isolates shared 94.0% and 96.5% identity at nucleotide and amino acid level, respectively. They shared 94.5–94.9% and 92.5–92.9% homology with S8 of RBSDV Japanese isolate at nucleotide and amino acid level, respectively; shared 85.1–87.6% and 91.7–91.9% homology with S7 of Italian MRDV (maize rough dwarf virus).
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Project (RC 9604) supported by Natural Science Foundation of Zhejiang Province, China.
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Zhang, Hm., Chen, Jp., Xue, Qz. et al. cDNA cloning and sequence analysis of genome segments S8 from rice black-streaked dwarf virus. J. Zheijang Univ.-Sci. A 3, 216–222 (2002). https://doi.org/10.1631/BF03396441
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DOI: https://doi.org/10.1631/BF03396441