Abstract
Objective: to explore the frequency and significance of ApoE gene polymorphisms in Chinese patients with atherosclerotic cerebral infarction (ACI). Methods: Polymerase chain reaction and gene sequencing, single nucleotide polymorphisms of ApoE gene were used to analyze 33 cases of patients with ACI and 35 controls. Results: The frequencies of ApoE gene single nucleotide polymorphisms 465C/G, 462C/G and 451 delC in the ACI group were significantly higher than those in the control group (P<0.05). The prevalence of polymorphism 486G/T in the control group was significantly higher than that in the ACI group (P=0.011). Conclusions: 465C/G, 462C/G and 451 delC polymorphisms might be associated with ACI. 486GT allale might have protective effect on the pathogenesis of ACI.
References
Kitagawa, K., Matsumoto, M., Kuwabara, K., Ohtsuki, T. and Hori, M., 2001. Delayed, but marked, expression of apolipoprotein E is involved in tissue clearance after cerebral infarction.J Cereb Blood Flow Metab,21 (10): 1199–1207.
MacLeod, M. J., De Lange, R. P., Breen, G., Meiklejohn, D., Lemmon, H. and Clair, D. S., 2001. Lack of association between apolipoprotein E genoype and ischaemic stroke in a Scottish population.Eur J Clin Invest,31 (7): 570–573.
Martin, E. R., Lai, E. H., Gilbert, J. R., Rogala, A. R., Afshari, A. J., Riley, J., Finch, K. L., Stevens, J. F., Livak, K. J., Slotterbeck, B. D., Slifer, S. H., Warren, L. L., Conneally, P. M., Schmechel, D. E., Purvis, I., Pericak-Vance, M. A., Roses, A. D. and Vance, J. M., 2000. SNPing away at complex disease: analysis of single-nucleotide polymorphisms around ApoE in Alzheimer disease.Am J Hum Genet,67(2): 383–394.
McCarron, M. O., Delong, D. and Alberts, M. J., 1999. ApoE genotype as a risk factor for ischemic cerebrovascular disease: a meta-analysis.Neurology,53(6): 1308–1311.
McLean, J. W., Elshourbagy, N. A., Chang, D. J., Mahley, R. W. and Taylor, J. M., 1984. Human apolipoprotein E mRNA. cDNA cloning and nucleotide sequencing of a new variant.J Biol Chem,259(10): 6498–6504.
Siest, G., Pillot, T., Regis-Bailly, A., Leininger-Muller, B., Steinmetz, J., Galteau, M. M. and Visvikis, S., 1995. Apolipoprotein E: an important gene and protein to follow in laboratory medicine.Clin. Chem,418: 1068–1086.
Wang, D. G., Fan, J. B., Siao, C. J., Berno, A., Young, P., Sapolsky, R., Ghandour, G., Perkins, N., Winchester, E., Spencer, J., Kruglyak, L., Stein, L., Hsie, L., Topaloglou, T., Hubbell, E., Robinson, E., Mittmann, M., Morris, M. S., Shen, N., Kilburn, D., Rioux, J., Nusbaum, C., Rozen, S., Hudson, T. J. and Lander, E. S., 1998. Large-Scale identification, mapping, and genotyping of single-nucleotide polymorphisms in the human genome.Science,280(5366): 1077–1082.
Author information
Authors and Affiliations
Corresponding author
Additional information
Project (No. 2000B076) supported by the Bureau of Public rlealth of Zhejiang Province
Rights and permissions
About this article
Cite this article
Ben-yan, L., Zhi, C., Feng, C. et al. Study of apolipoprotein E genetic polymorphism in patients with atherosclerotic cerebral infarction. J. Zheijang Univ.-Sci. 4, 749–752 (2003). https://doi.org/10.1631/BF02851619
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1631/BF02851619