Abstract
Analyses of the correlation between hyperspectral reflectance and pigment content including chlorophyll-a, chlorophyll-b and carotenoid of leaves in different sites of rice were reported in this paper. The hyperspectral reflectance of late rice during the whole growing season was measured using a Spectroradiometer with spectral range of 350–1050 nm and resolution of 3 nm. The chlorophyll-a, chlorophyll-b and carotenoid contents in rice leaves in rice fields to which different levels of nitrogen were applied were measured. The chlorophyll-a content of upper leaves was well correlated with the spectral variables. However, the correlation between both chlorophyll-b and caroteniod and the spectral variables was far from that of chlorophyll-a. The potential of hyperspectral reflectance measurement for estimating chlorophyll-a of upper leaves was evaluated using univariate correlation and multivariate regression analysis methods with different types of predictors. This study showed that the most suitable estimated model of chlorophyll-a of upper leaves was obtained by using some hyperspectral variables such asSD r,SD b and their integration.
References
Bai, B. Z. and Tang, X. J., 1993. Testing Technology of Plant Physiology. China Science and Technology Press, Beijing, China (in Chinese).
Blackburn, G. A., 1998. Spectral indices for estimating photosynthectic pigment concentrations: A test using senescent tree leaves.International Journal of Remote Sensing,19(4): 657–675.
Card, D. H., Peterson, D. L. and Matson, P. A., 1988. Prediction of leaf chemistry by the use of visible and near infrared reflectance spectroscopy.Remote Sensing of Environment,26(2): 123–147.
Carter, G. A., 1998. Reflectance bands and indices for remote estimation of photosynthesis and stomatal conductance in pine canopies.Remote Sensing of Environment,63(1): 61–72.
Chappelle, E. W., Kim, M. S. and mcMurtrey, J. E., 1992. Ratio analysis of reflectance spectra (RARS): an algorithm for the remote estimation of the concentrations of chlorophylla, chlorophyllb and the carotenoid in soybean leaves.Remote Sensing of Environment,39(3): 239–247.
Chen, Z., Elvidge, C. D. and Groeneveld, D. P., 1998. Monitoring seasonal dynamics of arid land vegetation using AVIRIS data.Remote Sensing of Environment,65 (3): 255–266.
Dawson, T. P., Curran, P. J., North, P. R. J. and Plummer, S. E., 1999. The propagation of foliar biochemical absoption features in forest canopy reflectance: a theoretical analysis.Remote Sensing of Environment,67(2): 147–159.
Goetz, S. J. and Prince, S. D., 1996. Remote sensing of net primary production in boreal forest sands.Agricultural and Forest Meteorology,78(3–4): 149–179.
Gong, P., Pu, R. and Miller, J. R., 1995. Coniferous forest leaf area index estimation along the Oregon transact using compact airborne spectrographic image data.Photogrammetric Engineering and Remote Sensing,61 (9): 1107–1117.
Penuelas, J., Baret, F. and Filella, I., 1995. Semi-empirical indices to assess carotenoids/chlorophylla ratio from leaf spectral reflectance.Photosynthetica,31(1): 221–230.
Pu, R. and Gong, P., 2000. Hyperspectral Remote Sensing and its Applications. Higher Education Press, Beijing, China, p. 205–206 (in Chinese).
Thomas, J. R. and Gausman, H. W., 1977. Leaf reflectance versus leaf chlorophyll and carotenoid concentration for eight crops.agronomy journal,69(2): 799–802.
Yoder, B. J. and Pettigrew-Crosby, R. E., 1995. Predicting nitrogen and chlorophyll content and concentrations from reflectance spectra (400–2500nm) at leaf and canopy scales.Remote Sensing of Environment,53(3): 199–211.
Author information
Authors and Affiliations
Corresponding author
Additional information
Project (No. 40171065) supported by the National Natural Science Foundation of China.
Rights and permissions
About this article
Cite this article
Qian, C., Jing-feng, H., Xiu-zhen, W. et al. In situ hyperspectral data analysis for pigment content estimation of rice leaves. J. Zheijang Univ.-Sci. 4, 727–733 (2003). https://doi.org/10.1631/BF02851615
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1631/BF02851615