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Study on the effect of doxorubicin on expressions of genes encoding myocardial sarcoplasmic reticulum Ca2+ transport proteins and the effect of taurine on myocardial protection in rabbits

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Abstract

To investigate the effect of doxorubicin(DOX) on gene expression of the myocardial sarcoplasmic reticulum (SR) Ca2+ transport proteins and the mechanism of taurine(Tau) protecting cardiac muscle cells, 9 rabbits were injected with DOX, 8 rabbits with DOX and Tau, and 9 rabbits with normal saline. Cardiac function, concentration of calcium in cardiomycocytes (Myo [Ca2+]i), activity of SR Ca2+-ATPase (SERCA2a), level of SERCA2a mRNA and Ca2+ released channels (RYR2) mRNA were detected. The left ventricle tissues were observed by electron microscopy. The results showed that cardiac index, left ventricular systolic pressure, activity of SR Ca2+-ATPase and level of SERCA2a mRNA decreased, while Myo[Ca2+]i increased in DOX-treated rabbits. DOX could not affect the level of RYR2 mRNA. Tau intervention could alleviate the increase of left ventricular diastolic pressure, Myo[Ca2+]i and the decrease of SERCA2a mRNA induced by doxorubicin. The results suggested that downregulation of SERCA2a gene expression was an important mechanism of DOX-induced cardiomyopathy and that Tau could partially improve the heart function by reducing calcium overload and alleviating downregulation of SERCA2a mRNA.

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Authors and Affiliations

  1. Children's Hospital, College of Medicine, Zhejiang University, 310003, Hangzhou, China

    Huang Xian-mei, Zhu Wei-hua & Kang Man-li

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  1. Huang Xian-mei
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  2. Zhu Wei-hua
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  3. Kang Man-li
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Correspondence to Huang Xian-mei.

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Xian-mei, H., Wei-hua, Z. & Man-li, K. Study on the effect of doxorubicin on expressions of genes encoding myocardial sarcoplasmic reticulum Ca2+ transport proteins and the effect of taurine on myocardial protection in rabbits. J. Zheijang Univ.-Sci. 4, 114–120 (2003). https://doi.org/10.1631/BF02841089

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  • DOI: https://doi.org/10.1631/BF02841089

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