Abstract
Two-component genes are kinds of genetic elements involved in regulation of antibiotic production inStreptomyces coelicolor. DNA microarray analysis revealed thatecrA1/A2, which mapped at distant sites fromred lucus and encode respectively the kinase and regulator, expressed coordinately with genes of Red specific biosynthetic pathway.ecrA1 andecrA2 gene-disruptive mutants were constructed using homogenotisation by reciprocal double crossover. Fermentation data showed that the undecylprodigiosin (Red) level of production was lower than that of wild-type strain. However, the change of the actinorhodin (Act) production level was not significant compared with wild type. Thus, these experiment results confirmed that the two-component systemecrA1/A2 was positive regulatory element forred gene cluster.
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Project (No.20172046) supported by the National Natural Foundation of China
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Yong-quan, L., Pei-lin, C., Shi-fei, C. et al. A pair of two-component regulatory genesecrA1/A2 inS. coelicolor. J. Zheijang Univ.-Sci. 5, 173–179 (2004). https://doi.org/10.1631/BF02840919
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DOI: https://doi.org/10.1631/BF02840919