Abstract
The authors investigated the influence of green tea polyphenols (GTPs) on the liver mitochondria permeability transition pore (PTP) opening through mitochondria swelling and change of mitochondria membrane potential. The data showed that GTPs had obvious protective effect on the Ca2+-induced PTP opening in a dose-dependent manner detected by mitochondria swelling. The results were obtained by measuring the change of mitochondria membrane potential through Rh 123. Further experiments were conducted to examine the detailed influence of GTPs on Ca2+ import and export of mitochondria. The results showed that GTPs had remarkably inhibitory effect on the Ca2+-induced Ca2+ import in mitochondria; and that they could accelerate Ca2+-release from mitochondria. Our data provide an alternate interpretation of the potent protective function of GTPs on cell against apoptosis.
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References
Ahmad, N., Feyes, D. K., Niemen, A. L. et al., 1997. Green tea constituent epigallocatechin-3-gallate and induction of apoptosis and cell cycle arrest in human carcinoma cells.J. Natl. Cancer Inst. 89: 1881–1886.
Ahmad, N., Mukhtar, H., 1999. Green tea polyphenols and cancer: biologic mechanisms and practical implications.Nutr. Rev. 57: 78–83.
Bushman, J. L., 1998. Green tea and cancer in humans: a review of the literature.Nutr. Cancer. 31: 151–159.
Desagher, S., Martinou, J. C., 2000. Mitochondria as the central control point of apoptosis.Trends in Cell Biology. 9: 369–377.
Fontaime, E., Eriksson, O., Ichas, F. et al., 1998. Regulation of the permeability transition pore in skeletal muscle mitochondria.J. Biol. Chem. 273(20): 12662.
Frei, B., Winterhalter, K. H., Richter, C., 1985. Mechanism of alloxan-induced calcium release from liver mitochondria.J. Biol. Chem. 260: 7394.
Gottlieb, R. A., 2000. Mitochondria: execution central.FEBS Letters. 82(1–2): 6–12.
Green, D. R., and Reed, J. C., 1998. Mitochondria and apoptosis.Science. 281: 1309–1312.
Ichas, F., Jouaville, L. S., Mazat, J. P. et al., 1997.Cell. 89: 1145–1153.
Islarn, S., Islam, N., Kermode, T. et al., 2000. Involvement of caspase-3 in epigallocatechin-3-gallate-mediated apoptosis of human chondrosarcoma cells.BBRC. 270: 793–797.
Jankun, J., Selman, S. H., Swiercz, R. et al., 1997. Why drinking green tea could prevent cancer.Nature. 387: 561.
Lemasters, J. J., Nieminen, T., Qian, L. C. et al., 1998. The mitochondrial permeability transition in cell death: a common mechanism in necrosis, apoptosis and autophagy.Biochem. Biophys. Acta. 1366: 177–196.
Lenaz, G., 1998. Role of mitochondria in oxidative stress and ageing.Biochem. Biophys. Acta. 1366: 52–67.
Luo, X., Budihardjo, I., Zou, H., Slaughter, C., Wang, X., 1998. e** Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors.Cell. 94: 481–490.
Pozzan, T., Rizzuto, R., 2000. The renaissance of mitochondrial calcium transport.Eur. J. Biochem. 267 (17): 5269–5273.
Steele, V. E., Bagheri, D., Balentine, D. A. et al., 1999. Preclinical efficacy studies of green and black tea extracts.Proc. Soc. Exp. Biol. Med. 220: 210–212.
Huang, X. X., Zhai, D. Y. 2000. Mitochondria Ca2+ transport and permeability transition pore opening and mitochondria energetic status.Science in China. 43 (5): 480–497.
Yang, G. Y., Liao, J., Kim, K. et al., 1998. Inhibition of growth and induction of apoptosis in human cancer cell lines by tea polyphenols.Carcinogenesis. 19 (4): 611–616.
Zoratti, M., Szabo, I., 1995. The mitochondria permeability transition. Biochem. Biophys. Acta.1241: 139–176.
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Sheng-rong, S., Hai-ning, Y. & Chao-fang, J. Influence of green tea polyphenols on mitochondrial permeability transition pore and Ca2+ transport. J. Zhejiang Univ.-Sci. 3, 475–479 (2002). https://doi.org/10.1631/BF02839493
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DOI: https://doi.org/10.1631/BF02839493