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Journal of Zhejiang University-SCIENCE B

, Volume 20, Issue 4, pp 332–342 | Cite as

Screening, purification, and characterization of an extracellular lipase from Aureobasidium pullulans isolated from stuffed buns steamers

  • Yang Li
  • Tong-jie Liu
  • Min-jie Zhao
  • Hui Zhang
  • Feng-qin FengEmail author
Article
  • 9 Downloads

Abstract

An extracellular lipase from Aureobasidium pullulans was obtained and purified with a specific activity of 17.7 U/mg of protein using ultrafiltration and a DEAE-Sepharose Fast Flow column. Characterization of the lipase indicated that it is a novel finding from the species A. pullulans. The molecular weight of the lipase was 39.5 kDa, determined by sodium dodecyl sulfonate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme exhibited its optimum activity at 40 °C and pH of 7. It also showed a remarkable stability in some organic solutions (30%, v/v) including n-propanol, isopropanol, dimethyl sulfoxide (DMSO), and hexane. The catalytic activity of the lipase was enhanced by Ca2+ and was slightly inhibited by Mn2+ and Zn2+ at a concentration of 10 mmol/L. The lipase was activated by the anionic surfactant SDS and the non-ionic surfactants Tween 20, Tween 80, and Triton X-100, but it was drastically inhibited by the cationic surfactant cetyl trimethyl ammonium bromide (CTAB). Furthermore, the lipase was able to hydrolyze a wide variety of edible oils, such as peanut oil, corn oil, sunflower seed oil, sesame oil, and olive oil. Our study indicated that the lipase we obtained is a potential biocatalyst for industrial use.

Key words

Lipase Aureobasidium pullulans Purification Enzymatic characterization 

一种产自出芽短梗霉的胞外脂肪酶的分离纯化和酶学性质研究

概 要

目 的

从出芽短梗霉所产的脂肪酶中筛选具有独特酶学 性质的脂肪酶

创新点

发现了一种新的产自出芽短梗霉的脂肪酶, 并对 其酶学性质进行了研究。

方 法

通过超滤和 DEAE-Sepharose Fast Flow 阴离子层 析柱方法对脂肪酶进行纯化, 随后分别用对硝基 酚邻酸盐(pNPP) 法对纯化得到的脂肪酶进行了 酶学性质研究, 并用酸碱中和法检测了脂肪酶对 可食用油脂的水解。

结 论

对分离纯化得到的脂肪酶的酶学性质研究表明, 该酶的分子量为39.5 kDa, 具有一个亚基, 为胞外酶。最佳催化温度为40 °C, 最佳催化pH 为7。该酶对一些有机溶剂、表面活性剂和离子具有优良的抗性。此外, 它可以水解常见的食用油。这些良好的特性使该脂肪酶有可能被应用于洗涤剂生产、生物柴油合成和食品制造等一些工业领域。

关键词

脂肪酶 出芽短梗霉 分离纯化 酶学性质表征 

CLC number

Q814.1 

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Copyright information

© Zhejiang University and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Biosystems Engineering and Food ScienceZhejiang UniversityHangzhouChina
  2. 2.School of Management and E-businessZhejiang Gongshang UniversityHangzhouChina
  3. 3.Zhejiang Key Laboratory for Agro-Food ProcessingZhejiang UniversityHangzhouChina

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