Journal of Zhejiang University-SCIENCE B

, Volume 20, Issue 2, pp 180–192 | Cite as

Oral administration of Lactobacillus rhamnosus GG to newborn piglets augments gut barrier function in pre-weaning piglets

  • Yang Wang
  • Li Gong
  • Yan-ping Wu
  • Zhi-wen Cui
  • Yong-qiang Wang
  • Yi Huang
  • Xiao-ping ZhangEmail author
  • Wei-fen LiEmail author


To understand the effects of Lactobacillus rhamnosus GG (ATCC 53103) on intestinal barrier function in pre-weaning piglets under normal conditions, twenty-four newborn littermate piglets were randomly divided into two groups. Piglets in the control group were orally administered with 2 mL 0.1 g/mL sterilized skim milk while the treatment group was administered the same volume of sterilized skim milk with the addition of viable L. rhamnosus at the 1st, 3rd, and 5th days after birth. The feeding trial was conducted for 25 d. Results showed that piglets in the L. rhamnosus group exhibited increased weaning weight and average daily weight gain, whereas diarrhea incidence was decreased. The bacterial abundance and composition of cecal contents, especially Firmicutes, Bacteroidetes, and Fusobacteria, were altered by probiotic treatment. In addition, L. rhamnosus increased the jejunal permeability and promoted the immunologic barrier through regulating antimicrobial peptides, cytokines, and chemokines via Toll-like receptors. Our findings indicate that oral administration of L. rhamnosus GG to newborn piglets is beneficial for intestinal health of pre-weaning piglets by improving the biological, physical, and immunologic barriers of intestinal mucosa.

Key words

Lactobacillus rhamnosus Gut microbiota Intestinal physical barrier Intestinal immunological barrier Piglet 

口服鼠李糖乳杆菌 GG 影响哺乳仔猪肠道屏障功能的研究

概 要

目 的

探究新生仔猪口服鼠李糖乳杆菌 GG 对肠道屏障功能的影响。


新生仔猪早期口服鼠李糖乳杆菌 GG 可明显改善其断奶前肠道菌群结构及免疫屏障功能。

方 法

二十四头新生仔猪分为对照组和实验组: 对照组仔猪在出生后第 1、 3、 5 天口服 2 mL 0.1 g/mL 的脱脂牛奶; 而实验组仔猪口服等体积的含有活鼠李糖乳杆菌 GG 的脱脂牛奶。 饲喂 25 天后, 收集仔猪血清、 肠道粘膜和盲肠内容物等样品。 通过分析肠道菌群、 紧密连接蛋白和细胞因子等指标, 评价鼠李糖乳杆菌对肠道屏障功能的影响。

结 论

在正常生理条件下, 新生仔猪口服鼠李糖乳杆菌 GG 可明显改变肠道菌群结构。 此外, 鼠李糖乳杆菌 GG 还可增加仔猪肠道的通透性, 并通过调控抗菌肽、 细胞因子和趋化因子的分泌以改善肠道的免疫屏障功能。


鼠李糖乳杆菌 肠道菌群 肠道物理屏障 肠道免疫屏障 仔猪 

CLC number


Supplementary material

11585_2019_331_MOESM1_ESM.pdf (211 kb)
Oral administration of Lactobacillus rhamnosus GG to newborn piglets augments gut barrier function in pre-weaning piglets


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Copyright information

© Zhejiang University and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Molecular Animal Nutrition and Feed Science, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, College of Animal ScienceZhejiang UniversityHangzhouChina
  2. 2.Department of Animal SciencesOregon State UniversityCorvallisUSA
  3. 3.College of Animal Science and TechnologyGuangxi UniversityNanningChina
  4. 4.China National Bamboo Research CenterKey Laboratory of High Efficient Processing of Bamboo of Zhejiang ProvinceHangzhouChina

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