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Journal of Zhejiang University SCIENCE B

, Volume 14, Issue 5, pp 382–391 | Cite as

Protective effect of hydrogen-rich saline on ischemia/reperfusion injury in rat skin flap

  • Ling Zhao
  • You-bin Wang
  • Shi-rui Qin
  • Xue-mei Ma
  • Xue-jun Sun
  • Ming-lian Wang
  • Ru-gang Zhong
Article

Abstract

Objective

Skin damage induced by ischemia/reperfusion (I/R) is a multifactorial process that often occurs in plastic surgery. The mechanisms of I/R injury include hypoxia, inflammation, and oxidative damage. Hydrogen gas has been reported to alleviate cerebral I/R injury by acting as a free radical scavenger. Here, we assessed the protective effect of hydrogen-rich saline (HRS) on skin flap I/R injury.

Methods

Abdominal skin flaps of rats were elevated and ischemia was induced for 3 h; subsequently, HRS or physiological saline was administered intraperitoneally 10 min before reperfusion. On postoperative Day 5, flap survival, blood perfusion, the accumulation of reactive oxygen species (ROS), and levels of cytokines were evaluated. Histological examinations were performed to assess inflammatory cell infiltration.

Results

Skin flap survival and blood flow perfusion were improved by HRS relative to the controls. The production of malondialdehyde (MDA), an indicator of lipid peroxidation, was markedly reduced. A multiplex cytokine assay revealed that HRS reduced the elevation in the levels of inflammatory cytokines, chemokines and growth factors, with the exception of RANTES (regulated on activation, normal T-cell expressed and secreted) growth factor. HRS treatment also reduced inflammatory cell infiltration induced by I/R injury.

Conclusions

Our findings suggest that HRS mitigates I/R injury by decreasing inflammation and, therefore, has the potential for application as a therapy for improving skin flap survival.

Key words

Hydrogen-rich saline Ischemia/reperfusion Skin flaps Oxidative damage Inflammation 

CLC number

Q74 R459.9 

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Copyright information

© Zhejiang University and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.College of Life Science and BioengineeringBeijing University of TechnologyBeijingChina
  2. 2.Peking Union Medical College HospitalBeijingChina
  3. 3.Department of Diving Medicine, Faculty of Naval Medicinethe Second Military Medical UniversityShanghaiChina

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