Journal of Zhejiang University SCIENCE B

, Volume 12, Issue 2, pp 116–125 | Cite as

Microarray-based identification of tomato microRNAs and time course analysis of their response to Cucumber mosaic virus infection

  • Qiu-lei Lang
  • Xiao-chuan Zhou
  • Xiao-lin Zhang
  • Rafal Drabek
  • Zhi-xiang Zuo
  • Yong-liang Ren
  • Tong-bin Li
  • Ji-shuang Chen
  • Xiao-lian Gao
Article

Abstract

A large number of plant microRNAs (miRNAs) are now documented in the miRBase, among which only 30 are for Solanum lycopersicum (tomato). Clearly, there is a far-reaching need to identify and profile the expression of miRNAs in this important crop under various physiological and pathological conditions. In this study, we used an in situ synthesized custom microarray of plant miRNAs to examine the expression and temporal presence of miRNAs in the leaves of tomato plants infected with Cucumber mosaic virus (CMV). Following computational sequence homology search and hairpin structure prediction, we identified three novel tomato miRNA precursor genes. Our results also show that, in accordance with the phenotype of the developing leaves, the tomato miRNAs are differentially expressed at different stages of plant development and that CMV infection can induce or suppress the expression of miRNAs as well as up-regulate some star miRNAs (miRNA*s) which are normally present at much lower levels. The results indicate that developmental anomalies elicited by virus infection may be caused by more complex biological processes.

Key words

Solanum lycopersicum Plant miRNA Cucumber mosaic virus miRNA array miRNA response 

CLC number

Q945.8 

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Supplementary material

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Copyright information

© Zhejiang University and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Qiu-lei Lang
    • 1
    • 2
  • Xiao-chuan Zhou
    • 3
  • Xiao-lin Zhang
    • 3
  • Rafal Drabek
    • 4
  • Zhi-xiang Zuo
    • 3
  • Yong-liang Ren
    • 3
  • Tong-bin Li
    • 3
  • Ji-shuang Chen
    • 1
    • 2
  • Xiao-lian Gao
    • 3
    • 4
  1. 1.College of Life SciencesZhejiang UniversityHangzhouChina
  2. 2.Institute of BioengineeringZhejiang Sci-Tech UniversityHangzhouChina
  3. 3.LC SciencesHoustonUSA
  4. 4.Department of Biology and BiochemistryUniversity of HoustonHoustonUSA

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