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Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29

Abstract

An antifungal protein was isolated from a culture of Bacillus subtilis strain B29. The isolation procedure comprised ion exchange chromatography on diethylaminoethyl (DEAE)-52 cellulose and gel filtration chromatography on Bio-Gel® P-100. The protein was absorbed on DEAE-cellulose and Bio-Gel® P-100. The purified antifungal fraction was designated as B29I, with a molecular mass of 42.3 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), pI value 5.69 by isoelectric focusing (IEF)-PAGE, and 97.81% purity by high performance liquid chromatography (HPLC). B29I exhibited inhibitory activity on mycelial growth in Fusarium oxysporum, Rhizoctonia solani, Fusarium moniliforme, and Sclerotinia sclerotiorum. The 50% inhibitory concentrations (IC50) of its antifungal activity toward Fusarium oxysporum and Rhizoctonia solani were 45 and 112 μmol/L, respectively. B29I also demonstrated an inhibitory effect on conidial spore germination of Fusarium oxysporum and suppression of germ-tube elongation, and induced distortion, tumescence, and rupture of a portion of the germinated spores.

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Author information

Correspondence to Qian Yang.

Additional information

Project supported by the Hi-Tech Research and Development Program (863) of China (No. 2003AA241140) and the Natural Science Foundation of Heilongjiang Province, China (No. C200522)

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Li, J., Yang, Q., Zhao, L. et al. Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29. J. Zhejiang Univ. Sci. B 10, 264–272 (2009) doi:10.1631/jzus.B0820341

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Key words

  • Bacillus subtilis
  • Antifungal protein
  • Purification

CLC number

  • Q81