Sensing Escherichia coli O157:H7 via frequency shift through a self-assembled monolayer based QCM immunosensor
By means of the specific immuno-recognition and ultra-sensitive mass detection, a quartz crystal microbalance (QCM) biosensor for Escherichia coli O157:H7 detection was developed in this work. As a suitable surfactant, 16-mercaptohexadecanoic acid (MHDA) was introduced onto the Au surface of QCM, and then self-assembled with N-hydroxysuccinimide (NHS) raster as a reactive intermediate to provide an active interface for the specific antibody immobilization. The binding of target bacteria with the immobilized antibodies decreased the sensor’s resonant frequency, and the frequency shift was correlated to the bacterial concentration. The stepwise assembly of the immunosensor was characterized by means of the electrochemical techniques. Using the immersion-dry-immersion procedure, this QCM biosensor could detect 2.0×102 colony forming units (CFU)/ml E. coli O157:H7. In order to reduce the fabrication time, a polyelectrolyte layer-by-layer self-assembly (LBL-SA) method was adopted for fast construction. Finally, the reproducibility of this biosensor was discussed.
Key wordsBiosensor Escherichia coli O157:H7 Immunosensor Layer-by-layer self-assembly (LBL-SA) Quartz crystal microbalance (QCM)
Unable to display preview. Download preview PDF.
- CDC (Centers for Disease Control and Prevention), 2006. Ongoing Multistate Outbreak of Escherichia coli Serotype O157:H7 Infections Associated with Consumption of Fresh Spinach. US. http://www.cdc.gov.mill1.sjlibrary.org/mmwr/preview/mmwrhtml/mm55d926a1.htm
- Kikuchi, M., Shiratori, S., 2005. Quartz crystal microbalance (QCM) sensor for CH3SH gas by using polyelectrolyte-coated sol-gel film. Sensors and Actuators B Chemical, 108(1–2):564–571. [doi:10.1016/j.snb.2004.12.122]Google Scholar
- Magliulo, M., Simoni, P., Guardigli, M., Michelini, E., Luciani, M., Lelli, R., Roda, A., 2007. A rapid multiplexed chemiluminescent immunoassay for the detection of Escherichia coli O157:H7, Yersinia enterocolitica, Salmonella typhimurium, and Listeria monocytogenes pathogen bacteria. J. Agric. Food Chem., 55(13):4933–4939. [doi:10.1021/jf063600b]PubMedCrossRefGoogle Scholar
- Tu, S.I., Uknalis, J., Irwin, P., Yu, L.S.L., 2000. The use of strepavidin coated magnetic beads for detecting pathogenic bacteria by light addressable potentionmetric sensor. J. Rapid Meth. Automat. Microbiol., 8(4):96–109.Google Scholar
- Tuttle, J., Gomez, T., Doyle, M.P., Wells, J.G., Zhao, T., Tauxe, R.V., Griffin, P.M., 1999. Lessons from a large outbreak of Escherichia coli O157:H7 infections: Insights into the infectious dose and method of widespread contamination of hamburger patties. Epidemiol. Infect., 122(2):185–192. [doi:10.1017/S0950268898001976]PubMedCrossRefGoogle Scholar
- Varshney, M., Li, Y., 2007. Interdigitated array microelectrode based impedance biosensor coupled with magnetic nanoparticle-antibody conjugates for detection of Escherichia coli O157:H7 in food samples. Biosens. Bioelectron., 22(11):2408–2414. [doi:10.1016/j.bios.2006.08.030]PubMedCrossRefGoogle Scholar