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Journal of Zhejiang University SCIENCE B

, Volume 7, Issue 10, pp 817–824 | Cite as

Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum

  • Zheng Yi-xiong 
  • Ringe Jochen 
  • Liang Zhong 
  • Loch Alexander 
  • Chen Li 
  • Sittinger Michael 
Article

Abstract

The use of periosteum-derived progenitor cells (PCs) combined with bioresorbable materials is an attractive approach for tissue engineering. The aim of this study was to characterize the osteogenic differentiation of PC in 3-dimensional (3D) poly-lactic-co-glycolic acid (PLGA) fleeces cultured in medium containing allogeneic human serum. PCs were isolated and expanded in monolayer culture. Expanded cells of passage 3 were seeded into PLGA constructs and cultured in osteogenic medium for a maximum period of 28 d. Morphological, histological and cell viability analyses of three-dimensionally cultured PCs were performed to elucidate osseous synthesis and deposition of a calcified matrix. Furthermore, the mRNA expression of type I collagen, osteocalcin and osteonectin was semi-quantitively evaluated by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). The fibrin gel immobilization technique provided homogeneous PCs distribution in 3D PLGA constructs. Live-dead staining indicated a high viability rate of PCs inside the PLGA scaffolds. Secreted nodules of neo-bone tissue formation and the presence of matrix mineralization were confirmed by positive von Kossa staining. The osteogenic differentiation of PCs was further demonstrated by the detection of type I collagen, osteocalcin and osteonectin gene expression. The results of this study support the concept that this tissue engineering method presents a promising method for creation of new bone in vivo.

Key words

Tissue engineering Poly-lactic-co-glycolic acid polymer Periosteum-derived progenitor cells 3-dimensional culture 

CLC number

Q819 

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Copyright information

© Zhejiang University 2006

Authors and Affiliations

  • Zheng Yi-xiong 
    • 1
  • Ringe Jochen 
    • 2
  • Liang Zhong 
    • 2
  • Loch Alexander 
    • 3
  • Chen Li 
    • 1
  • Sittinger Michael 
    • 2
  1. 1.Department of Surgery, the Second Affiliated Hospital, School of MedicineZhejiang UniversityHangzhouChina
  2. 2.Tissue Engineering Laboratory, Department of Rheumatology and Clinical ImmunologyCharité-University MedicineBerlinGermany
  3. 3.Department of OtorhinolaryngologyCharité-University MedicineBerlinGermany

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