Journal of Zhejiang University-SCIENCE A

, Volume 7, Supplement 2, pp 366–370

Improvement of spinosad producing Saccharopolyspora spinosa by rational screening

  • Jin Zhi-hua 
  • Wu Jian-ping 
  • Zhang Yuan 
  • Cheng Xiu 
  • Yang Li-rong 
  • Cen Pei-lin 
Article

DOI: 10.1631/jzus.2006.AS0366

Cite this article as:
Jin, Z., Wu, J., Zhang, Y. et al. J. Zhejiang Univ. - Sci. A (2006) 7(Suppl 2): 366. doi:10.1631/jzus.2006.AS0366

Abstract

Spinosad (spinosyns A and D) is a mixture of secondary metabolites produced by Saccharopolyspora spinosa. It is used in agriculture as a potent insect control agent with exceptional safety to non-target organisms. Spinosyns are macrolides with a 21-carbon, tetracyclic lactone backbone to which the deoxysugars forosamine and tri-O-methylrhamnose are attached. According to the pathway and regulation of spinosad biosynthesis, a rational selection procedure with u.v. mutation was performed to obtain high spinosad producing strain. The spinosad resistant mutants, the rhamnose resistant mutants, the 2-deoxygen-D-glucose resistant mutants were selected, successively. Eventually, a strain S. spinosa 4∼6 was obtained, with its production of spinosad reaching 268 mg/L, which is increased by 121% in comparison with that of the parent strain S. spinosa 1∼5. The subculture experiments indicated that the hereditary character of high production of S. spinosa 4∼6 is stable. The spinosad fermentation with S. spinosa 4∼6 was scaled up in a 10 L fermentor, and a production of 458 mg/L was obtained, which was 71% higher than the production with shaking-flask fermentation.

Key words

Spinosad Saccharopolyspora spinosa Strain improvement Rational screening 

CLC number

Q815 

Copyright information

© Zhejiang University 2006

Authors and Affiliations

  • Jin Zhi-hua 
    • 1
    • 2
  • Wu Jian-ping 
    • 2
  • Zhang Yuan 
    • 2
  • Cheng Xiu 
    • 2
  • Yang Li-rong 
    • 2
  • Cen Pei-lin 
    • 2
  1. 1.Department of Biological and Pharmaceutical Engineering, Ningbo Institute of TechnologyZhejiang UniversityNingboChina
  2. 2.Institute of Bioengineering, School of Materials Science and Chemical EngineeringZhejiang UniversityHangzhouChina

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