Abstract
An in vitro study of the viability of normal human osteoblasts (NHOst) cultured on poly (3-hydroxybutyrate), PHB, scaffolds was conducted. PHB was biosynthesized from a strain of Azotobacter vinelandii, and the NHOst were cultured on cast films and on electrospun membranes. The cell viability was investigated for up to 168 h and parallel studies were carried out on control (empty) wells. The cell concentration increased exponentially with time and after 168 h. The concentration was still increasing although at lower rate and the cell nuclei were still active. The osteoblast viability and morphology were both healthy in both type of scaffolds, and after 168 h. The viability was over 90% regardless of the scaffold morphology. This in vitro study utilizing PHB derived from A. vinelandii strains suggests that the scaffolds are a feasible alternative to bone tissue regeneration and warrants an in vivo study.
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The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Supplementary file Supporting materials and methods: physical-chemical characterization, thermal analysis via TGA and DSC, morphology characterization by SEM, WAXD and optical microscopy, scaffolds fabrication, cell culturing and quantification; cell viability assessment (PDF). (PDF 687 kb)
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Romo-Uribe, A. Human osteoblast cells proliferation in biodegradable poly-3-hydroxybutyrate (PHB) scaffolds from a mutant Azotobacter vinelandii strain. MRS Advances 7, 508–514 (2022). https://doi.org/10.1557/s43580-022-00286-3
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DOI: https://doi.org/10.1557/s43580-022-00286-3