Abstract
A crucial balance between oxidative eustress and distress is important for maintaining redox homeostasis in the cellular milieu. Therefore, sustaining the intracellular redox buffer condition with exogenous agents could be a therapeutic strategy against diseases caused by redox imbalance. Here, we synthesized chitosan-functionalized Mn3O4 nanoparticles (Ch-Mn3O4 NPs) and tested their redox buffering capability in in-vitro and in-cellulo. Chitosan is easily absorbed by the intestine and can be used as a target-specific delivery agent to the intestine, while Mn3O4 NPs have redox modulatory properties. Therefore, combination of chitosan and Mn3O4 NPs provide the opportunity for targeted redox buffering. Targeted delivery of a drug and remediation of the corresponding indication qualify the drug to be a theranostic agent. Our spectroscopic studies suggest ROS generation as well as antioxidant ability of Ch-Mn3O4 NPs. In-cellulo studies using A549 cell lines confirmed the efficacy of the nanohybrid in redox homeostasis. The outcomes suggest that Ch-Mn3O4 nanohybrid has the potential to function as a target-specific redox buffering agent in both in-vitro and in-cellulo systems.
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Acknowledgments
MD thanks University Grants Commission (UGC), Govt. of India for Junior Research Fellowship. SKP thanks the Indian National Academy of Engineering (INAE) for the Abdul Kalam Technology Innovation National Fellowship, INAE/121/AKF. The authors thank the DBT (WB)-BOOST scheme for the financial grant, 339/WBBDC/1P-2/2013. The authors would like to thank Dr. Uttam Pal for helping in graphical abstract preparation.
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Mondal, S., Adhikari, A., Ghosh, R. et al. Synthesis and spectroscopic characterization of a target-specific nanohybrid for redox buffering in cellular milieu. MRS Advances 6, 427–433 (2021). https://doi.org/10.1557/s43580-021-00087-0
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DOI: https://doi.org/10.1557/s43580-021-00087-0