We investigate the single-molecule detection of anodic corrosion redox reactions of iron using two fluorophores, FeRhoNox-1 and FluoZin-3, which “turn-on” upon reacting with Fe2+. Both dye molecules show potential as fluorogenic sensors for detecting anodic corrosion of iron in an aqueous environment, but FeRhoNox-1 shows a larger change in fluorescence signal than FluoZin-3. Deviations from the ensemble observations of iron corrosion are observed when performing single-molecule counting analysis of the collected images of FeRhoNox-1 “turning-on” over time. A complete picture of the corrosion initiation at the molecular scale can be obtained by combining the Fe2+-sensitive detection with cathodic corrosion reaction detection.
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The data that support the findings of this study are available from the corresponding authors upon reasonable request.
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The authors would like to thank the laboratory of Prof. Strangi at the Case Western Reserve University for use of their fluorimeter. The authors thank the Case Western Reserve University College of Arts and Sciences for financial support and members of the Kisley Research Group for helpful discussion.
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The authors declare that there are no known conflicts of interest.
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Saini, A., Gatland, Z., Begley, J. et al. Investigation of fluorophores for single-molecule detection of anodic corrosion redox reactions. MRS Communications (2021). https://doi.org/10.1557/s43579-021-00096-y