In order to improve bacterial antifouling property, titanium (Ti) surfaces were functionalized through the plasma polymerization (PP) technique with diethyl allyl phosphate (DAP) as amphoteric precursor. Antifouling properties of the surfaces were evaluated in vitro by Escherichia coli (ATCC® 25922™) and Staphylococcus aureus (ATCC® 25923™) strain, and results showed that 75.00% and 81.25% bacterial reduction achieved on functionalized surfaces, respectively. In vitro cell culture studies were carried out with BJ normal human skin fibroblast (ATCC® CRL-2522™) cell line. These findings revealed that amphoteric plasma polymer prepared from DAP offers promising solution for preventing Biofilm formation on Ti.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
S. Liao, C. Chang, C. Chen, C. Lee, W. Lin, Functionalization of pure titanium MAO coatings by surface modifications for biomedical applications. Surf. Coat. Technol. (2020). https://doi.org/10.1016/j.surfcoat.2020.125812
Z.M. Ebimobowei, A. Emmanuel, A.J. Adegbindin, Mechanical strengthening and microstructural evolutions of Ni-B based hardfacing alloys influenced by titanium additions. Adv. Mater. 8, 41–47 (2019). https://doi.org/10.11648/j.am.20190802.11
S. Noumbissi, A. Scarano, S. Gupta, A literature review study on atomic ions dissolution of titanium and its alloys in implant dentistry. Materials. 12, 368 (2019). https://doi.org/10.3390/ma12030368
D.J. Stickler, Bacterial biofilms in patients with indwelling urinary catheters. Nat. Clin. Pract. Urol. 5, 598–608 (2008)
S. Noimark, C.W. Dunnill, M. Wilson, I.P. Parkin, The role of surfaces in catheter-associated infections. Chem. Soc. Rev. 38, 3435–3448 (2009)
H. Wang, H. Wang, Z. Wang, L. Tang, G. Zeng, P. Xu, M. Chen, J. Tang, Covalent organic framework photocatalysts: structures and applications. Chem. Soc. Rev. 49, 4135–4165 (2020)
C. Wu, Y. Zhou, M. Xu, P. Han, L. Chen, J. Chang, Y. Xiao, Copper-containing mesoporous bioactive glass scaffolds with multifunctional properties of angiogenesis capacity, osteostimulation and antibacterial activity. Biomaterials 34, 422–433 (2013)
M. Lucke, G. Schmidmaier, S. Sadoni, B. Wildemann, R. Schiller, N.P. Haas, M. Raschke, Gentamicin coating of metallic implants reduces implant-related osteomyelitis in rats. Bone 32, 521–531 (2003)
K.C. Popat, L. Leoni, C.A. Grimes, T.A. Desai, Influence of engineered titania nanotubular surfaces on bone cells. Biomaterials 28, 3188–3197 (2007)
K.C. Popat, M. Eltgroth, T.J. LaTempa, C.A. Grimes, T.A. Desai, Decreased Staphylococcus epidermis adhesion and increased osteoblast functionality on antibiotic-loaded titania nanotubes. Biomaterials 28, 4880–4888 (2007)
S. Radin, P. Ducheyne, Controlled release of vancomycin from thin sol–gel films on titanium alloy fracture plate material. Biomaterials 28, 1721–1729 (2007)
A.A. Campbell, L. Song, X.S. Li, B.J. Nelson, C. Bottoni, D.E. Brooks, E.S. DeJong, Development, characterization, and anti-microbial efficacy of hydroxyapatite-chlorhexidine coatings produced by surface-induced mineralization. J. Biomed. Mater. Res. 53, 400–407 (2000)
A. Nanci, J.D. Wuest, L. Peru, P. Brunet, V. Sharma, S. Zalzal, M.D. McKee, Chemical modification of titanium surfaces for covalent attachment of biological molecules. J. Biomed. Mater. Res. 40, 324–335 (1998)
H.F. Özgüzar, G.K. Can, G. Kabay, M. Mutlu, Quartz tuning fork as a mass sensitive biosensor platform with a bi-layer film modification via plasma polymerization. MRS Commun. 9, 710–718 (2019)
A.E. Meydan, H.F. Özgüzar, J.S. Göçmen, M. Mutlu, Controlled Drug Release Performance of Plasma Modified Slab and Mat Matrices: A Model Study with “Ampicillin.” Int. J. Pharm. 587, 119586 (2020)
G.K. Can, H.F. Özgüzar, G. Kabay, P. Kömürcü, M. Mutlu, Simultaneous insulation and modification of quartz tuning fork surface by single-step plasma polymerization technique with amine-rich precursors. MRS Commun. 8, 541–549 (2018)
E. Akdogan, M. Demirbilek, Y. Sen, M.A. Onur, O.K. Azap, E. Sonmez, M. Mutlu, In vitro and in vivo bacterial antifouling properties of phosphite plasma-treated silicone. Surf. Innovat. 7, 122–132 (2019)
C.A. Streuli, Determination of basicity of substituted phosphines by nonaqueous titrimetry. Anal. Chem. 32, 985–987 (1960)
G. Kaleli-Can, H.F. Özgüzar, S. Kahriman, M. Türkal, J.S. Göçmen, E. Yurtçu, M. Mutlu, Improvement in antimicrobial properties of titanium by diethyl phosphite plasma-based surface modification. Mater. Today Commun. 25, 101565 (2020)
E. Akdoğan, M. Mutlu, Generation of Amphoteric surfaces via glow-discharge technique with single precursor and the behavior of bovine serum albumin at the surface. Colloids Surf. B 89, 289–294 (2012)
G. Kaleli-Can, E. Hortaç-İştar, H.F. Özgüzar, M. Mutlu, H.C. Mirza, A. Başustaoğlu, J.S. Göçmen, Prevention of Candida biofilm formation over polystyrene by plasma polymerization technique. MRS Commun. 10, 667–673 (2020)
M. Bryjak, I. Gancarz, G. Poźniak, Surface evaluation of plasma-modified polysulfone (Udel P-1700) films. Langmuir 15, 6400–6404 (1999)
JIS, Z. 2801: 2000 Antimicrobial Products: Test for Antimicrobial Activity and Efficacy. Japanese Standards Association, Tokyo
Y. Wu, J.P. Zitelli, K.S. TenHuisen, X. Yu, M.R. Libera, Differential response of Staphylococci and Osteoblasts to varying titanium surface roughness. Biomaterials 32, 951–960 (2011)
J. Yu, N. Xu, Y. Xi, M. Xue, W. Wang, X. Huang, X. Ye, Adhesion behavior of Escherichia coli on plasma-sprayed Zn and Ag Co-incorporated calcium silicate coatings with varying surface roughness. J. Therm. Spray Technol. 27, 1428–1435 (2018)
S. Guo, D. Jańczewski, X. Zhu et al., Surface charge control for zwitterionic polymer brushes: tailoring surface properties to antifouling applications. J. Colloid Interface Sci. 452, 43–53 (2015)
R. Yang, P. Moni, K.K. Gleason, Ultrathin zwitterionic coatings for roughness independent underwater superoleophobicity and gravity driven oil water separation. Adv. Mater. Interfaces 2, 1400489 (2015)
M.W. Sabaa, N.A. Mohamed, R.R. Mohamed, N.M. Khalil, S.M. Abd El Latif, Synthesis, characterization and antimicrobial activity of poly (N-vinyl imidazole) grafted carboxymethyl chitosan. Carbohyd. Polym. 79, 998–1005 (2010)
H. Tu, G. Wu, Y. Yi, M. Huang, R. Liu, X. Shi, H. Deng, Layer-by-layer immobilization of amphoteric carboxymethyl chitosan onto biocompatible silk fibroin nanofibrous mats. Carbohyd. Polym. 210, 9–16 (2019)
J. Gallo, M. Holinka, C.S. Moucha, Antibacterial surface treatment for orthopaedic implants. Int. J. Mol. Sci. 15, 13849–13880 (2014). https://doi.org/10.3390/ijms150813849
R. Kugler, O. Bouloussa, F. Rondelez, Evidence of a charge-density threshold for optimum efficiency of biocidal cationic surfaces. Microbiol. Sgm. 151, 1341–1348 (2005). https://doi.org/10.1099/mic.0.27526-0
Below is the link to the electronic supplementary material.
About this article
Cite this article
Özgüzar, H.F., Meydan, A.E., Göçmen, J.S. et al. Single-step amphoteric surface modification through plasma polymerization: Antifouling coating for titanium substrate. MRS Communications 11, 523–531 (2021). https://doi.org/10.1557/s43579-021-00071-7