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Double-emulsion templated lipid vesicles as minimal cell mimics for assembling tissue-like vesicular materials

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Abstract

Lipid vesicles formed using double-emulsion drops as templates exhibit uniform sizes and compositions. Despite these important advantages, conventional electroformation continues to be the selected approach for vesicle fabrication to understand biophysical processes in cells using simplified model systems. Here, we address critical aspects that could be hindering the extensive use of emulsion-templating strategies for vesicle fabrication and emphasize certain systematic studies that would help demonstrate further the advantages of microfluidic technologies. Besides the importance of controlling size and composition, we envision that the high throughput of this technology will allow the construction of vesicular materials with controlled architectures.

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Abbreviations

CAM:

Cell adhesion molecule

DOPC:

1,2-Dioleoyl-sn-glycero-3-phosphocholine

DPPC:

1,2-Dipalmitoyl-sn-glycero-3-phosphocholine

DOPE-biotinyl:

1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine-N-(biotinyl) (sodium salt)

IVTT:

In vitro Transcription-translation

RICM:

Reflection interference contrast microscopy

W/O:

Water-in-oil

W/O/W:

Water-in-oil-in-water

W/O/O/W:

Water-in-oil-in-oil-in-water

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Acknowledgments

LRA and JLA acknowledge financial support from the Spanish Ministry of Science and Innovation (MCI), through the María de Maeztu Programme for Units of Excellence in R&D (CEX2018-000805-M), and through the project (RTI2018-101953-A-I00; MCI/AEI/FEDER, UE). LRA also acknowledges financial support from MCI through the Ramón y Cajal Programme (RYC2018-025575-I; MCI/AEI/FSE, UE).

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  1. Department of Theoretical Condensed Matter Physics, Condensed Matter Physics Center and Instituto Nicolás Cabrera, Universidad Autónoma de Madrid, 28049, Madrid, Spain

    Berta Tinao, Paula Magrinya, Juan L. Aragones & Laura R. Arriaga

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  1. Berta Tinao
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Correspondence to Laura R. Arriaga.

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Tinao, B., Magrinya, P., Aragones, J.L. et al. Double-emulsion templated lipid vesicles as minimal cell mimics for assembling tissue-like vesicular materials. MRS Communications 11, 18–30 (2021). https://doi.org/10.1557/s43579-020-00002-y

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  • DOI: https://doi.org/10.1557/s43579-020-00002-y

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