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Electrospun dimethyloxallylglycine sustained release scaffold for promoting the migration and multidirectional differentiation of stem cells from the apical papilla

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Abstract

Dental pulpitis, a common dental disease, poses a significant challenge for modern dentistry because of the intricate framework and limited regenerative capacity of dental pulp. Traditional pulp revascularization techniques have limitations in tissue regeneration and control over the type of regenerated tissue. The objective of this investigation is to investigate the capability of an innovative nanofiber tissue engineering scaffold for the regeneration of dental pulp, thereby delving into its latent possibilities. The scaffold, composed of polylactic acid-glycolic acid copolymer (PLGA) and dimethyloxalylglycine-Mesoporous silica nanoparticles (DMOG@MSNs), was fabricated using electrospinning technology. The DMOG@MSNs-PLGA scaffold exhibited good hydrophilicity, biocompatibility, and prolonged liberation of DMOG. In vitro assays demonstrated the scaffold promoted the proliferation, migration, and multidirectional differentiation of stem cells from apical papilla. The results indicate that DMOG@MSNs-PLGA scaffold holds promise for dental pulp regeneration, offering a potential strategy for regenerative endodontic therapy.

Trial registration number: JNSKQYY-2022-001, date of registration: March 9, 2022.

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Acknowledgments

The authors thank Peng Yan (College of Basic Medical Sciences, Binzhou Medical University) for providing an electrospinning machine.

Funding

This work was supported by the “Clinical + X” scientific and technological innovation project of Binzhou Medical University (BY2021LCX08); Natural Science Foundation of Shandong Province (ZR2019BH040); Ji Nan Science & Technology Bureau (202134005); Research Assistance Fund for the President of Ji Nan Stomatological Hospital.

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  1. Chen Li and Linqun Luan have equally contributed to this work and share first authorship.

Authors and Affiliations

  1. Institute of Stomatology, Binzhou Medical University, No.346 Guanhai Street, Yantai, 264003, Shandong, China

    Chen Li, Linqun Luan, Sainan Wang, Shuang Xiao & Li Zhang

  2. Department of Central Laboratory, Jinan Key Laboratory of Oral Tissue Regeneration & Shandong Provincial Health Commission Key Laboratory of Oral Diseases and Tissue Regeneration, Jinan Stomatological Hospital, Jinan, China

    Chen Li, Ao Lyu, Xiaonan Yu, Xijiao Yu & Yi Du

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Contributions

CL: methodology, formal analysis, writing-original draft. LL: investigation, formal analysis. AL: guidance of experimental techniques. XY: disinfection of experimental materials. SW: data curation. SX: data curation. XY: assistance of project administration, co-supervision, funding acquisition. LZ: funding acquisition, project administration, review & editing. YD: conceptualization, funding acquisition, project administration.

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Correspondence to Xijiao Yu, Li Zhang or Yi Du.

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The authors declare that they have no known conflicts of interest/competing interests or personal relationships that could have appeared to influence the work reported in this paper.

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This study was performed in line with the principles of the Declaration of Helsinki. Ethics approval was obtained from Jinan Stomatological Hospital for this study (Date of registration: March 9, 2022. Ethics approval number: JNSKQYY-2022-001).

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Li, C., Luan, L., Lyu, A. et al. Electrospun dimethyloxallylglycine sustained release scaffold for promoting the migration and multidirectional differentiation of stem cells from the apical papilla. Journal of Materials Research 39, 609–625 (2024). https://doi.org/10.1557/s43578-023-01253-w

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