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Fibroin reinforced, strontium-doped calcium phosphate silicate cements for bone tissue engineering applications

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Abstract

Calcium phosphate silicate cements (CPSCs) have been developed to overcome problems like high acidity due to presence of brushite and high temperatures evolving as polymethyl methacrylate (PMMA) cements cure at surgery site. However, CPSCs with good handling and injectability have not been successfully produced. Here, we aimed to develop a biocompatible and osteoconductive bone cement composed of strontium (Sr)-doped tri-calcium silicates (C3S), monocalcium monophosphate and silk fibroin (SF). C3S powders were mixed with monocalcium monophosphate, and SF to obtain injectable CPSC. Physical, mechanical and biological characterization studies revealed that SF presence minimized alkalinity, increased rate of weight loss, porosity and elasticity but decreased maximum yield strength. The in vitro cell culture studies showed that all CPSCs were biocompatible. CPSC-Sr2-2% SF group performed the best in terms of osteogenic differentiation. These results suggest that CPSCs hold promise as bone cement however, in vivo studies should be conducted.

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Data Availability

The data that support the findings of this study are available from the corresponding author, [AT], upon reasonable request.

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Acknowledgments

The authors acknowledge BIOMATEN-Center of Excellence in Biomaterials and Tissue Engineering and Central Laboratory of Middle East Technical University. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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  1. Department of Biotechnology, Middle East Technical University, Ankara, 06800, Turkey

    Gülhan Işık & Ayşen Tezcaner

  2. Department of Engineering Sciences, Middle East Technical University, Ankara, 06800, Turkey

    Ahmet Engin Pazarçeviren, Zafer Evis & Ayşen Tezcaner

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  1. Gülhan Işık
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Işık, G., Pazarçeviren, A.E., Evis, Z. et al. Fibroin reinforced, strontium-doped calcium phosphate silicate cements for bone tissue engineering applications. Journal of Materials Research 38, 5017–5031 (2023). https://doi.org/10.1557/s43578-023-01211-6

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