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Mesoporous silica application as an antidote of methotrexate and evaluation of the long-term oral administration: In vitro and in vivo study

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Abstract

Amino-functionalized mesoporous silica (MS-NH2) was synthesized, characterized, and applied as an adsorbent and antidote agent of methotrexate (MTX) in vitro and in vivo assessment. Different techniques, such as FT-IR and XRD, analyzed the structure. The texture parameters were determined by the N2 adsorption/desorption technique. The morphology was examined using FE-SEM. Prepared materials showed to have high porosity and nano-sized pores. The capacity of synthesized material in the adsorption of MTX was checked. The in vivo experiments were performed on mice that were overdosed with MTX. Serum biomarkers were assessed, and it was discovered that MS-NH2 administration effectively decreased MTX toxicity. Furthermore, in vivo evaluation was conducted for the long-term administration of MS-NH2 in the animal model. Analysis of plasma biomarkers showed no significant changes in organ injury after long-term administration. Cytotoxicity and LDH assay showed that MS-NH2 did not impose toxicity and damage in the studied cell line.

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Funding

The funding of this project (95-01-36-13763) was supported by the Research Council of Shiraz University of Medical Sciences.

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Authors and Affiliations

  1. Pharmaceutical Sciences Research Center, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran

    Reza Heidari, Zeinab Sepahi, Soliman Mohammadi-Samani & Fatemeh Farjadian

  2. Department of Pharmaceutics, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran

    Soliman Mohammadi-Samani

  3. School of Dentistry, Marquette University, Milwaukee, USA

    Lobat Tayebi

  4. Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

    Negar Azarpira

  5. Department of Cell and Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

    Mohammad Doroudian

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  1. Reza Heidari
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Contributions

FF and RH participated in the conceptualization, ZS and RH participated in the data acquisition, FF, ZS, and NA participated in the data analysis, SM-S, LT, and MD were consultants & performed data analysis. All authors contributed in writing of the manuscript.

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Correspondence to Fatemeh Farjadian.

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Animals were handled according to the SUMS guidelines for the care and use of laboratory animals approved by a local ethics committee at SUMS, Shiraz, Iran (Ethical code # IR.SUMS.REC.1396.S18).

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Heidari, R., Sepahi, Z., Mohammadi-Samani, S. et al. Mesoporous silica application as an antidote of methotrexate and evaluation of the long-term oral administration: In vitro and in vivo study. Journal of Materials Research 38, 2930–2942 (2023). https://doi.org/10.1557/s43578-023-01003-y

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