Abstract
Layered Double Hydroxide (LDHs) requires surface functionalization to achieve desirable colloidal and biological stability for effective drug delivery. The present work reported a new functionalized approaches to construct drug-loaded nanohybrids (2:1E and 2.8:1E) by co-assembling LDH sheets, penta-fluorouracil (5Fu) and poloxamer 188 through exfoliation reassembling. Some analysis showed that more poloxamer 188 were needed to prepare 2:1E with appropriate particle size than 2.8:1E. Besides, 2.8:1E shows advantages in drug loading rate and dispersion as compared with ion exchange method. In addition, these nanohybrids can improve the sudden release of 5Fu in naked drug-loaded LDH. Most importantly, significant enhanced cell uptake and better cytotoxicity were seen in these nanohybrids in contrast to naked drug-loaded LDH. Lastly, the results reveal that assembling drug-loaded LDH with poloxamer 188 is a promising strategy to improve cellular uptake and in vitro efficacy of LDHs for biomedical applications.
Graphical abstract
Drug-loaded nanohybrids were synthesised by co-assembling LDH sheets, penta-fluorouracil (5Fu) and poloxamer 188 through exfoliation reassembling, and showed advantages in dispersion, enhanced cell uptake and better cytotoxicity as compared with naked drug-loaded-LDH prepared by ion exchange method.
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We declare that all data generated or analyzed during this study are included in this published article; and the data are available from the corresponding author on reasonable request.
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The authors thank to the National Natural Science Foundation of China [81860631, 21967013, 21867013], Scientific Research Fund of Jiangxi Provincial Health Commission [20201118], and Natural Science Foundation of Jiangxi Province (20192ACBL20032).
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Ren, J., Liang, L., Yang, Y. et al. Assembling drug-loaded-layered double hydroxide nanohybrids with poloxamer 188 for improved cellular uptake and in vitro efficacy. Journal of Materials Research 38, 337–349 (2023). https://doi.org/10.1557/s43578-022-00813-w
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DOI: https://doi.org/10.1557/s43578-022-00813-w