Abstract
In this study, magnesium and strontium-doped β-tricalcium phosphates were synthesized to understand dopant impact on substrate chemistry, morphology, proliferation, and osteogenic differentiation of mesenchymal stem cells. Under solid-state synthesis, magnesium doping stabilized the β-phase in tricalcium phosphate, with 22% less α-phase content than control. Strontium doping increased α-phase formation by 17%, and also resulted in greater surface porosity, leading to greater crystal precipitation in vitro. Magnesium also significantly enhanced the proliferation of stem cells (P < 0.05) and differentiation into osteoblasts with increased alkaline phosphatase production (P < 0.05) at all time points. These results indicated that magnesium stabilizes β-tricalcium phosphate in vitro and enhanced early and late-time-point osteoconduction and osteoinduction of mesenchymal stem cells.
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Acknowledgments
The authors would like to thank the financial support from the National Institutes of Health, NIH National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS-R01-AR-066361) and NIH National Institute of Dental and Cranofacial Research (NIDCR-R01-DE-029204-A1).The authors also would like to acknowledge the Franceschi Microscopy and Imaging Center at Washington State University.
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Susmita Bose was an editor of this journal during the review and decision stage. For the JMR policy on review and publication of manuscripts authored by editors, please refer to http://www.mrs.org/editor-manuscripts/.
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Vahabzadeh, S., Robertson, S. & Bose, S. Beta-phase stabilization and increased osteogenic differentiation of stem cells by solid-state synthesized magnesium tricalcium phosphate. Journal of Materials Research 36, 3041–3049 (2021). https://doi.org/10.1557/s43578-021-00311-5
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DOI: https://doi.org/10.1557/s43578-021-00311-5