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Hydroxyapatite-coated liposomes for the controlled release of quantum dots and bupivacaine

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Abstract

This work reports the development of hydroxyapatite-coated liposomes to encapsulate drug bupivacaine and quantum dots (CdSe) to be used as a theranostic tool. Systems like this allow monitoring drug distribution in vivo (because of the quantum dots) and they can modulate the rate of drug delivery by increasing drug concentration in situ (due to the hydroxyapatite coating). Liposomes were prepared by the injection technique, and their hydroxyapatite coating was obtained by co-precipitation of calcium and phosphate precursors. The increase in diameter and decrease in zeta potential of the hydroxyapatite-coated liposomes (LHAP) when compared to conventional liposomes evidenced that a core–shell was formed around the vesicles. X-ray diffraction and infrared spectroscopy measurements confirmed the presence of hydroxyapatite in the coating. The liposomes were then functionalized with CdSe to be used as theranostic and detected by photoluminescence. The bupivacaine was incorporated in liposomes/hydroxyapatite and its in vitro release kinetics was modulated, showing slower rates than those measured with the conventional liposomes.

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Acknowledgments

The authors are thankful to the Spectroscopy laboratory at the State University of Londrina (UEL), and Labmult—Laboratory of Technological Federal University of Paraná, Londrina, PR, for use of their facilities. The capes-ds scholarship program (Grant Number 1795788).

Funding

This study was funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes-ds program) (grant number 1795788).

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Authors and Affiliations

  1. Materials Engineering Department - Technological, Federal University of Paraná, Londrina, PR, Brazil

    Felipe Ferreira Lopes & Marcio Florian

  2. Chemistry Department, State University of Maringá, Maringá, PR, Brazil

    Camila Fabiano de Freitas

  3. Biochemistry and Tissue Biology Department, Biology Institute, University of Campinas, Campinas, SP, Brazil

    Eneida de Paula

  4. Physics Department - Technological, Federal University of Paraná, Londrina, PR, Brazil

    Sidney Alves Lourenço

  5. Department of Chemistry, Technological Federal University of Parana (UTFPR), Avenida dos Pioneiros, 3131, Londrina, PR, CEP 86036-370, Brazil

    Luis Fernando Cabeça

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  1. Felipe Ferreira Lopes
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  2. Camila Fabiano de Freitas
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  3. Eneida de Paula
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  4. Sidney Alves Lourenço
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Correspondence to Luis Fernando Cabeça.

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Lopes, F.F., de Freitas, C.F., de Paula, E. et al. Hydroxyapatite-coated liposomes for the controlled release of quantum dots and bupivacaine. Journal of Materials Research 36, 3021–3030 (2021). https://doi.org/10.1557/s43578-021-00292-5

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