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Recent advances in personalized 3D bioprinted tissue models

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Abstract

Three-dimensional (3D) bioprinting uses the defined layer-by-layer deposition of living cells incorporated into biocompatible materials that can be used to create 3D models of human tissues. Functional 3D in vitro models can better mimic the complex architecture of human tissues in vivo providing more accurate cell-to-cell and cell-to-matrix interactions, and a better supply of nutrients, oxygen, and drugs to cells than standard two-dimensional cultures. This article examines recent advances and employments of these personalized models in cardiac, cancer, skin, and neuronal tissue applications based on the use of 3D printing and patient-derived cells, including induced pluripotent stem cells. These models can be used to generate patient-specific organ prototypes, drug screening platforms in preclinical studies, and engraftable tissues suitable for clinical practice proving themselves as promising new avenues for disease modeling, drug discovery, and regenerative medicine.

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Adapted with permission from (a) Reference 21. (b, c) Reproduced under the Creative Commons Attribution 4.0 license for Reference 24. © 2016 Termedia & Banach.

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Adapted with permission from (a) Reference 34, (b, c) Reference 39, (d) Reference 40. Reproduced under the Creative Commons Attribution 4.0 license for References 34 and 39 and © 2018 Wiley for Reference 40.

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Adapted with permission from (a, b) Reference 47, (c) Reference 48. © 2022 Royal Society of Chemistry for Reference 47 and © 2022 The Authors. Published by Wolters Kluwer Health, Inc. on behalf of The American Society of Plastic Surgeons for Reference 48.

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Adapted with permission from (a, b) Reference 49, (c–f) Reference 51, (g, h) Reference 56. © 2017 Wiley for Reference 49. Reproduced under the Creative Commons Attribution 4.0 license for Reference 51 and © 2016 Wiley for Reference 56.

Figure 6

Adapted with permission from (a) Reference 73, (b–d) Reference 71, (e–g) Reference 72, (h–l) Reference 61. Reproduced under the Creative Commons Attribution 4.0 license for Reference 73. © 2018 Wiley for Reference 71, © 2021 Elsevier for Reference 72, and © 2020 Wiley for Reference 61.

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The funding for this article was provided by the Canada Research Chairs Program (Tier II Chair in Biomedical Engineering), Natural Sciences and Engineering Research Council Discovery Grant Program, and the Canadian Institutes of Health Research - Institute of Neurosciences, Mental Health and Addiction Project Grant program.

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The idea for this article was S.M.W. The literature search was performed by J.P.W.-S. and S.S. J.P.W.-S. and S.S. drafted this work. S.M.W. and H.B.N. made critical revisions.

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Correspondence to Haakon B. Nygaard or Stephanie M. Willerth.

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S. Willerth is the CEO of Axolotl Biosciences, a startup focused on selling novel bioinks for bioprinting human tissue models. The remaining authors declare that there is no conflict of interest.

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Walters-Shumka, J.P., Sorrentino, S., Nygaard, H.B. et al. Recent advances in personalized 3D bioprinted tissue models. MRS Bulletin 48, 632–642 (2023). https://doi.org/10.1557/s43577-023-00551-2

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