Abstract
A de novo drug delivery nanosystem based on gold nanoparticles (GNPs), decorated poly(ethylene glycol) (PEG), and folate (FA)-conjugated graphene oxide (GO) was designed and developed successfully. Initially, the graphite (G) powder was oxidized to the GO, and then functionalized with chloroacetic acid to afford a carboxylated graphene oxide (GO–COOH). The obtained GO–COOH was functionalized with an amine end-caped PEG, FA, as well as 3-amino-1-propanethiol to produce a GO–PEG–FA–SH. In another experimental section, GNPs were synthesized through a citrate-mediated reduction approach, and subsequently decorated onto/into GO–PEG–FA–SH through the formation of Au–S bond to afford a GO–PEG–FA/GNP nanosystem. The resultant nanosystem was loaded with doxorubicin hydrochloride (DOX) as a model anticancer drug, and its drug-loading capacity as well as pH-dependent drug release behavior were investigated. The anticancer activity of the developed theranostic nanomedicine was extensively evaluated using MTT assay against human breast cancer cells (MCF7). The developed GO–PEG–FA/GNPs–DOX theranostic nanomedicine exhibited an excellent cancer chemotherapy feature. In addition, this nanomedicine can be used in chemo-photothermal therapy of solid tumors because of the presence of GO and GNPs in its structure.
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Acknowledgments
The authors gratefully acknowledge the partial financial support from Nano Drug Delivery Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran (Grant No. 980305), and Payame Noor University, Tehran, Iran.
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Samadian, H., Mohammad-Rezaei, R., Jahanban-Esfahlan, R. et al. A de novo theranostic nanomedicine composed of PEGylated graphene oxide and gold nanoparticles for cancer therapy. Journal of Materials Research 35, 430–441 (2020). https://doi.org/10.1557/jmr.2020.3
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DOI: https://doi.org/10.1557/jmr.2020.3